The mRNA transportome of the BicD / Egl transport machinery

Vazquez Pianzola, Maria Paula; Schaller, Bogdan; Colombo, Martino; Beuchle, Dirk; Neuenschwander, Samuel; Marcil, Anne; Bruggmann, Rémy; Suter, Beat (2017). The mRNA transportome of the BicD / Egl transport machinery. RNA biology, 14(1), pp. 73-89. Taylor and Francis 10.1080/15476286.2016.1251542

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Messenger RNA (mRNA) transport focuses the expression of encoded proteins to specific regions within cells providing them with the means to assume specific functions and even identities. BicD and the mRNA binding protein Egl interact with the microtubule motor dynein to localize mRNAs in Drosophila. Because relatively few mRNA cargos were known, we isolated and identified Egl::GFP associated mRNAs. The top candidates were validated by qPCR, in situ hybridization and genetically by showing that their localization requires BicD. In young embryos these Egl target mRNAs are preferentially localized apically, between the plasma membrane and the blastoderm nuclei, but also in the pole plasm at the posterior pole. Egl targets expressed in the ovary were mostly enriched in the oocyte and some were apically localized in follicle cells. The identification of a large group of novel mRNAs associated with BicD/Egl points to several novel developmental and physiological functions of this dynein dependent localization machinery. The verified dataset also allowed us to develop a tool that predicts conserved A'-form-like stem loops that serve as localization elements in 3'UTRs.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Departement of Chemistry and Biochemistry
08 Faculty of Science > Department of Biology > Institute of Cell Biology

UniBE Contributor:

Vazquez Pianzola, Maria Paula; Colombo, Martino; Beuchle, Dirk; Neuenschwander, Samuel; Bruggmann, Rémy and Suter, Beat

Subjects:

500 Science > 570 Life sciences; biology
500 Science > 540 Chemistry

ISSN:

1555-8584

Publisher:

Taylor and Francis

Funders:

[4] Swiss National Science Foundation
[80] Kanton Bern

Language:

English

Submitter:

Beat Suter

Date Deposited:

23 Jan 2017 14:52

Last Modified:

23 Jan 2017 14:52

Publisher DOI:

10.1080/15476286.2016.1251542

PubMed ID:

27801632

BORIS DOI:

10.7892/boris.92278

URI:

https://boris.unibe.ch/id/eprint/92278

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