Delivery of membrane proteins into small and giant unilamellar vesicles by charge-mediated fusion

Biner, Olivier Felix; Schick, Thomas; Müller, Yannic; von Ballmoos, Christoph (2016). Delivery of membrane proteins into small and giant unilamellar vesicles by charge-mediated fusion. FEBS letters, 590(14), pp. 2051-2062. Wiley 10.1002/1873-3468.12233

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One of the current challenges in synthetic biology is the production of stable membrane mimetic systems and the insertion of components in these systems. Here, we employ fusion of oppositely charged liposomes to deliver separately reconstituted membrane proteins into a common lipid bilayer. After a systematic evaluation of different lipid compositions by lipid mixing and size distribution analysis, suitable conditions were further investigated for proteoliposome fusion. With this technique, we functionally coreconstituted bo3 oxidase and ATP synthase from Escherichia coli into unilamellar liposomes ranging from 100 nm to 50 μm in size. The presented method is a simple and versatile tool for oriented membrane protein reconstitution to produce biomimetic systems with increased complexity.

Item Type:

Journal Article (Further Contribution)

Division/Institute:

08 Faculty of Science > Departement of Chemistry and Biochemistry

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Biner, Olivier Felix; Schick, Thomas and von Ballmoos, Christoph

Subjects:

500 Science > 570 Life sciences; biology
500 Science > 540 Chemistry

ISSN:

0014-5793

Publisher:

Wiley

Language:

English

Submitter:

Olivier Felix Biner

Date Deposited:

24 Jan 2017 10:24

Last Modified:

26 Jan 2017 06:03

Publisher DOI:

10.1002/1873-3468.12233

PubMed ID:

27264202

Uncontrolled Keywords:

charged lipids giant unilamellar vesicle liposomes membrane fusion protein reconstitution respiratory chain enzymes

BORIS DOI:

10.7892/boris.92412

URI:

https://boris.unibe.ch/id/eprint/92412

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