Primary Postnatal Dorsal Root Ganglion Culture from Conventionally Slaughtered Calves.

Fadda, Angela; Bärtschi, Michelle; Hemphill, Andrew; Widmer, Hans Rudolf; Zurbriggen, Andreas; Perona, P; Vidondo Curras, Beatriz; Oevermann, Anna (2016). Primary Postnatal Dorsal Root Ganglion Culture from Conventionally Slaughtered Calves. PLoS ONE, 11(12), e0168228. Public Library of Science 10.1371/journal.pone.0168228

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Neurological disorders in ruminants have an important impact on veterinary health, but very few host-specific in vitro models have been established to study diseases affecting the nervous system. Here we describe a primary neuronal dorsal root ganglia (DRG) culture derived from calves after being conventionally slaughtered for food consumption. The study focuses on the in vitro characterization of bovine DRG cell populations by immunofluorescence analysis. The effects of various growth factors on neuron viability, neurite outgrowth and arborisation were evaluated by morphological analysis. Bovine DRG neurons are able to survive for more than 4 weeks in culture. GF supplementation is not required for neuronal survival and neurite outgrowth. However, exogenously added growth factors promote neurite outgrowth. DRG cultures from regularly slaughtered calves represent a promising and sustainable host specific model for the investigation of pain and neurological diseases in bovines.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > NeuroCenter
05 Veterinary Medicine > Research Foci > Veterinary Public Health / Herd Health Management
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > DKV - Clinical Neurology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Parasitology
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV)
04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Neurosurgery
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Veterinary Public Health Institute
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Experimental Clinical Research
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH)

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Fadda, Angela; Bärtschi, Michelle; Hemphill, Andrew; Widmer, Hans Rudolf; Zurbriggen, Andreas; Vidondo Curras, Beatriz and Oevermann, Anna

Subjects:

600 Technology > 610 Medicine & health
600 Technology > 630 Agriculture

ISSN:

1932-6203

Publisher:

Public Library of Science

Language:

English

Submitter:

Nicole Söll

Date Deposited:

26 Jan 2017 15:01

Last Modified:

11 Oct 2018 16:58

Publisher DOI:

10.1371/journal.pone.0168228

PubMed ID:

27936156

BORIS DOI:

10.7892/boris.92425

URI:

https://boris.unibe.ch/id/eprint/92425

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