Direct determination of phosphate sugars in biological material by 1H high-resolution magic-angle-spinning NMR spectroscopy

Diserens, Gaëlle; Vermathen, Martina; Gjuroski, Ilche; Eggimann, Sandra; Precht, Maria Christina; Boesch, Christoph Hans; Vermathen, Peter (2016). Direct determination of phosphate sugars in biological material by 1H high-resolution magic-angle-spinning NMR spectroscopy. Analytical and bioanalytical chemistry, 408(20), pp. 5651-5656. Springer 10.1007/s00216-016-9671-0

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The study aim was to unambiguously assign nucleotide sugars, mainly UDP-X that are known to be important in glycosylation processes as sugar donors, and glucose-phosphates that are important intermediate metabolites for storage and transfer of energy directly in spectra of intact cells, as well as in skeletal muscle biopsies by 1H high-resolution magic-angle-spinning (HR-MAS) NMR. The results demonstrate that sugar phosphates can be determined quickly and non-destructively in cells and biopsies by HR-MAS, which may prove valuable considering the importance of phosphate sugars in cell metabolism for nucleic acid synthesis. As proof of principle, an example of phosphate-sugar reaction and degradation kinetics after unfreezing the sample is shown for a cardiac muscle, suggesting the possibility to follow by HR-MAS NMR some metabolic pathways.

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