Direct determination of phosphate sugars in biological material by 1H high-resolution magic-angle-spinning NMR spectroscopy

Diserens, Gaëlle; Vermathen, Martina; Gjuroski, Ilche; Eggimann, Sandra; Precht, Maria Christina; Boesch, Christoph Hans; Vermathen, Peter (2016). Direct determination of phosphate sugars in biological material by 1H high-resolution magic-angle-spinning NMR spectroscopy. Analytical and bioanalytical chemistry, 408(20), pp. 5651-5656. Springer 10.1007/s00216-016-9671-0

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The study aim was to unambiguously assign nucleotide sugars, mainly UDP-X that are known to be important in glycosylation processes as sugar donors, and glucose-phosphates that are important intermediate metabolites for storage and transfer of energy directly in spectra of intact cells, as well as in skeletal muscle biopsies by 1H high-resolution magic-angle-spinning (HR-MAS) NMR. The results demonstrate that sugar phosphates can be determined quickly and non-destructively in cells and biopsies by HR-MAS, which may prove valuable considering the importance of phosphate sugars in cell metabolism for nucleic acid synthesis. As proof of principle, an example of phosphate-sugar reaction and degradation kinetics after unfreezing the sample is shown for a cardiac muscle, suggesting the possibility to follow by HR-MAS NMR some metabolic pathways.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > NeuroCenter
04 Faculty of Medicine > Department of Radiology, Neuroradiology and Nuclear Medicine (DRNN) > Institute of Diagnostic, Interventional and Paediatric Radiology > DCR Magnetic Resonance Spectroscopy and Methodology (AMSM)
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV)
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > DKV - Clinical Radiology
08 Faculty of Science > Departement of Chemistry and Biochemistry
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Pavillon 52 > Abt. Magnetresonanz-Spektroskopie und Methodologie, AMSM

UniBE Contributor:

Diserens, Gaëlle; Vermathen, Martina; Gjuroski, Ilche; Precht, Maria Christina; Boesch, Christoph Hans and Vermathen, Peter

Subjects:

500 Science > 570 Life sciences; biology
500 Science > 540 Chemistry
600 Technology > 630 Agriculture

ISSN:

1618-2642

Publisher:

Springer

Funders:

[4] Swiss National Science Foundation

Language:

English

Submitter:

Martina Vermathen

Date Deposited:

24 Jan 2017 11:02

Last Modified:

13 Jun 2018 11:03

Publisher DOI:

10.1007/s00216-016-9671-0

PubMed ID:

27271261

BORIS DOI:

10.7892/boris.92490

URI:

https://boris.unibe.ch/id/eprint/92490

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