In vitro diagnosis of T cell-mediated drug allergy

Porebski, G; Gschwend-Zawodniak, A; Pichler, W J (2011). In vitro diagnosis of T cell-mediated drug allergy. Clinical and experimental allergy, 41(4), pp. 461-70. Oxford: Blackwell Scientific Publications 10.1111/j.1365-2222.2011.03701.x

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Diagnosis of drug hypersensitivity relies on history, skin tests, in vitro tests and provocation tests. In vitro tests are of great interest, due to possible reduction of drug provocation tests. In this review we focus on best investigated in vitro techniques for the diagnosis of T cell-mediated drug hypersensitivity reactions. As drug hypersensitivity relies on different pathomechanisms and as a single diagnostic test usually does not cover all possible reactions, it is advisable to combine different tests to increase the overall sensitivity. Recently, proliferation-based assays have been supplemented by a panel of novel in vitro tests including analysis of cytotoxic potential of effector cells (granzyme B, granulysin, CD107a), evaluation of cytokine secretion (IL-2, IL-5, IL-13, and IFN-γ) and up-regulation of cell surface activation markers (CD69). We discuss the latest findings and readout systems to identify causative drugs by detecting functional and phenotypic markers of drug-reacting cells, and their ability to enable a more conclusive diagnosis of drug allergy.

Item Type:	Journal Article (Further Contribution)
Division/Institute:	04 Faculty of Medicine > Department of Dermatology, Urology, Rheumatology, Nephrology, Osteoporosis (DURN) > Clinic of Rheumatology and Immunology
UniBE Contributor:	Pichler, Werner Joseph
ISSN:	0954-7894
Publisher:	Blackwell Scientific Publications
Language:	English
Submitter:	Factscience Import
Date Deposited:	04 Oct 2013 14:26
Last Modified:	05 Dec 2022 14:07
Publisher DOI:	10.1111/j.1365-2222.2011.03701.x
PubMed ID:	21338430
Web of Science ID:	000288387200004
URI:	https://boris.unibe.ch/id/eprint/9323 (FactScience: 215022)