Copper resistance and its regulation in the sulfate reducing bacterium Desulfosporosinus sp. OT.

Mancini, Stefano; Kumar, Ranjeet; Abicht, Helge; Fischermeier, Elisabeth; Solioz, Marc (2016). Copper resistance and its regulation in the sulfate reducing bacterium Desulfosporosinus sp. OT. Microbiology, 162(4), pp. 684-693. Society for General Microbiology 10.1099/mic.0.000256

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Desulfosporosinus sp. OT is a Gram-positive, acidophilic sulfate-reducing Firmicute isolated from copper tailings sediment in the Norilsk mining-smelting area in Siberia and represents the first Desulfosporosinus species whose genome has been sequenced. Desulfosporosinus sp. OT is exceptionally copper resistant, which made it of interest to study the resistance mechanism. It possesses a copUAZ operon which is here shown to be involved in copper resistance. The copU gene encodes a CsoR-type homotetrameric repressor. By electrophoretic mobility shift assay, it was shown that CopU binds to the operator/promoter region of the copUAZ operon in the absence of copper, and is released from the DNA by Cu+ or Ag+, implying that CopU regulates the operon in a copper/silver-dependent manner. DOT_CopA is a P1B-type ATPase related to other characterized, bacterial copper ATPases. When expressed in a copper-sensitive Escherichia coli ΔcopA mutant, it restores copper resistance to wild-type levels. His-tagged DOT_CopA was expressed from a plasmid in E. coli and purified by Ni-NTA affinity chromatography. The purified enzyme is most active in the presence of Cu(I) and bacterial phospholipids. These findings indicate that the copUAZ operon confers copper resistance to Desulfosporosinus sp. OT, but do not per se explain the basis of the high copper resistance of this strain.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Hepatologie
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Hepatologie

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR)
04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Visceral Surgery and Medicine > Hepatology

UniBE Contributor:

Mancini, Stefano; Abicht, Helge and Solioz, Marc

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1350-0872

Publisher:

Society for General Microbiology

Language:

English

Submitter:

Lilian Karin Smith-Wirth

Date Deposited:

15 May 2017 10:37

Last Modified:

18 Jun 2017 11:08

Publisher DOI:

10.1099/mic.0.000256

PubMed ID:

26873027

BORIS DOI:

10.7892/boris.93610

URI:

https://boris.unibe.ch/id/eprint/93610

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