Development and validation of an immunohistochemistry procedure for the detection of a neurotropic bovine astrovirus

Boujon, Céline Louise; Selimovic-Hamza, Senija; Bouzalas, Ilias; Seuberlich, Torsten (2017). Development and validation of an immunohistochemistry procedure for the detection of a neurotropic bovine astrovirus. Journal of virological methods, 239, pp. 26-33. Elsevier 10.1016/j.jviromet.2016.10.013

[img]
Preview
Text
celines publication.pdf - Published Version
Available under License Creative Commons: Attribution-Noncommercial-No Derivative Works (CC-BY-NC-ND).

Download (1MB) | Preview

Members of the Astroviridae family are best known to cause diarrhea in different mammalian species. Lately, some strains have been associated with encephalitis in humans, minks and cattle. In this study, we developed an immunohistochemistry (IHC) procedure for the detection of a neurotropic bovine astrovirus (BoAstV-CH13/NeuroS1), which is associated with non-suppurative encephalitis in cattle. We expressed five recombinant antigens corresponding to different putative viral proteins of BoAstV-CH13/NeuroS1. Antigens were then used for the production of hyperimmune sera in rabbits. Out of the five hyperimmune sera, the one directed against the conserved N-terminus of the viral capsid protein, termed ORF2-con, clearly surpassed the others in the detection of viral antigens in IHC in terms of strong signal intensity and low background staining. The accuracy of the ORF2-con IHC protocol was then evaluated using different sets of brain tissue samples: 30 samples from 9 animals with confirmed BoAstV-CH13/NeuroS1 infection, 30 samples from 8 animals with non-suppurative encephalitis of another etiology and 30 samples from apparently healthy slaughtered animals. The IHC was positive only with tissue samples from animals with a known positive BoAstV-CH13/NeuroS1 status, but not with those from negative ones, indicating a good diagnostic sensitivity and specificity of the assay. The ORF2-con IHC procedure is therefore an adequate tool for the detection of BoAstV-CH13/NeuroS1 infections in cattle.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > NeuroCenter
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Experimental Clinical Research
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH)

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Boujon, Céline Louise; Selimovic-Hamza, Senija; Bouzalas, Ilias and Seuberlich, Torsten

Subjects:

500 Science
500 Science > 570 Life sciences; biology

ISSN:

0166-0934

Publisher:

Elsevier

Language:

English

Submitter:

Senija Selimovic-Hamza

Date Deposited:

18 Jul 2017 11:08

Last Modified:

06 Jun 2018 11:17

Publisher DOI:

10.1016/j.jviromet.2016.10.013

PubMed ID:

27916667

BORIS DOI:

10.7892/boris.94013

URI:

https://boris.unibe.ch/id/eprint/94013

Actions (login required)

Edit item Edit item
Provide Feedback