Link of a ubiquitous human coronavirus to dromedary camels.

Corman, Victor M; Eckerle, Isabella; Memish, Ziad A; Liljander, Anne M; Dijkman, Ronald; Jonsdottir, Hulda; Juma Ngeiywa, Kisi J Z; Kamau, Esther; Younan, Mario; Al Masri, Malakita; Assiri, Abdullah; Gluecks, Ilona; Musa, Bakri E; Meyer, Benjamin; Müller, Marcel A; Hilali, Mosaad; Bornstein, Set; Wernery, Ulrich; Thiel, Volker; Jores, Jörg; ... (2016). Link of a ubiquitous human coronavirus to dromedary camels. Proceedings of the National Academy of Sciences of the United States of America - PNAS, 113(35), pp. 9864-9869. National Academy of Sciences NAS 10.1073/pnas.1604472113

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The four human coronaviruses (HCoVs) are globally endemic respiratory pathogens. The Middle East respiratory syndrome (MERS) coronavirus (CoV) is an emerging CoV with a known zoonotic source in dromedary camels. Little is known about the origins of endemic HCoVs. Studying these viruses' evolutionary history could provide important insight into CoV emergence. In tests of MERS-CoV-infected dromedaries, we found viruses related to an HCoV, known as HCoV-229E, in 5.6% of 1,033 animals. Human- and dromedary-derived viruses are each monophyletic, suggesting ecological isolation. One gene of dromedary viruses exists in two versions in camels, full length and deleted, whereas only the deleted version exists in humans. The deletion increased in size over a succession starting from camelid viruses via old human viruses to contemporary human viruses. Live isolates of dromedary 229E viruses were obtained and studied to assess human infection risks. The viruses used the human entry receptor aminopeptidase N and replicated in human hepatoma cells, suggesting a principal ability to cause human infections. However, inefficient replication in several mucosa-derived cell lines and airway epithelial cultures suggested lack of adaptation to the human host. Dromedary viruses were as sensitive to the human type I interferon response as HCoV-229E. Antibodies in human sera neutralized dromedary-derived viruses, suggesting population immunity against dromedary viruses. Although no current epidemic risk seems to emanate from these viruses, evolutionary inference suggests that the endemic human virus HCoV-229E may constitute a descendant of camelid-associated viruses. HCoV-229E evolution provides a scenario for MERS-CoV emergence.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology
05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology

UniBE Contributor:

Dijkman, Ronald; Jonsdottir, Hulda Run; Thiel, Volker Earl and Jores, Jörg

Subjects:

600 Technology > 630 Agriculture

ISSN:

0027-8424

Publisher:

National Academy of Sciences NAS

Language:

English

Submitter:

Jörg Jores

Date Deposited:

01 Nov 2018 15:29

Last Modified:

01 Nov 2018 15:29

Publisher DOI:

10.1073/pnas.1604472113

PubMed ID:

27528677

Uncontrolled Keywords:

coronavirus ecology evolution livestock zoonotic diseases

BORIS DOI:

10.7892/boris.95441

URI:

https://boris.unibe.ch/id/eprint/95441

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