A conserved influenza A virus nucleoprotein code controls specific viral genome packaging.

Moreira, Étori Aguiar; Weber, Anna; Bolte, Hardin; Kolesnikova, Larissa; Giese, Sebastian; Lakdawala, Seema; Beer, Martin; Zimmer, Gert; García-Sastre, Adolfo; Schwemmle, Martin; Juozapaitis, Mindaugas (2016). A conserved influenza A virus nucleoprotein code controls specific viral genome packaging. Nature communications, 7(12861), p. 12861. Nature Publishing Group 10.1038/ncomms12861

[img]
Preview
Text
ncomms12861.pdf - Published Version
Available under License Creative Commons: Attribution (CC-BY).

Download (1MB) | Preview

Packaging of the eight genomic RNA segments of influenza A viruses (IAV) into viral particles is coordinated by segment-specific packaging sequences. How the packaging signals regulate the specific incorporation of each RNA segment into virions and whether other viral or host factors are involved in this process is unknown. Here, we show that distinct amino acids of the viral nucleoprotein (NP) are required for packaging of specific RNA segments. This was determined by studying the NP of a bat influenza A-like virus, HL17NL10, in the context of a conventional IAV (SC35M). Replacement of conserved SC35M NP residues by those of HL17NL10 NP resulted in RNA packaging defective IAV. Surprisingly, substitution of these conserved SC35M amino acids with HL17NL10 NP residues led to IAV with altered packaging efficiencies for specific subsets of RNA segments. This suggests that NP harbours an amino acid code that dictates genome packaging into infectious virions.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)

UniBE Contributor:

Zimmer, Gert

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 630 Agriculture

ISSN:

2041-1723

Publisher:

Nature Publishing Group

Language:

English

Submitter:

Achim Braun Parham

Date Deposited:

10 Jul 2017 08:56

Last Modified:

16 Jul 2017 02:14

Publisher DOI:

10.1038/ncomms12861

PubMed ID:

27650413

BORIS DOI:

10.7892/boris.96160

URI:

https://boris.unibe.ch/id/eprint/96160

Actions (login required)

Edit item Edit item
Provide Feedback