Post-entry blockade of small ruminant lentiviruses by wild ruminants.

San Jose Aranda, Leticia; Crespo, Helena; Blatti-Cardinaux, Laure Sarah Pauline; Glaria, Idoia; Martínez-Carrasco, Carlos; Berriatua, Eduardo; Amorena, Beatriz; De Andrés, Damián; Bertoni, Giuseppe; Reina, Ramses (2016). Post-entry blockade of small ruminant lentiviruses by wild ruminants. Veterinary research, 47(1), p. 1. BioMed Central 10.1186/s13567-015-0288-7

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Small ruminant lentivirus (SRLV) infection causes losses in the small ruminant industry due to reduced animal production and increased replacement rates. Infection of wild ruminants in close contact with infected domestic animals has been proposed to play a role in SRLV epidemiology, but studies are limited and mostly involve hybrids between wild and domestic animals. In this study, SRLV seropositive red deer, roe deer and mouflon were detected through modified ELISA tests, but virus was not successfully amplified using a set of different PCRs. Apparent restriction of SRLV infection in cervids was not related to the presence of neutralizing antibodies. In vitro cultured skin fibroblastic cells from red deer and fallow deer were permissive to the SRLV entry and integration, but produced low quantities of virus. SRLV got rapidly adapted in vitro to blood-derived macrophages and skin fibroblastic cells from red deer but not from fallow deer. Thus, although direct detection of virus was not successfully achieved in vivo, these findings show the potential susceptibility of wild ruminants to SRLV infection in the case of red deer and, on the other hand, an in vivo SRLV restriction in fallow deer. Altogether these results may highlight the importance of surveilling and controlling SRLV infection in domestic as well as in wild ruminants sharing pasture areas, and may provide new natural tools to control SRLV spread in sheep and goats.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology
05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

San Jose Aranda, Leticia; Blatti, Laure Sarah Pauline and Bertoni, Giuseppe

Subjects:

600 Technology > 630 Agriculture
500 Science > 570 Life sciences; biology

ISSN:

0928-4249

Publisher:

BioMed Central

Language:

English

Submitter:

Achim Braun Parham

Date Deposited:

07 Jul 2017 14:30

Last Modified:

09 Jul 2017 02:13

Publisher DOI:

10.1186/s13567-015-0288-7

PubMed ID:

26738942

BORIS DOI:

10.7892/boris.96164

URI:

https://boris.unibe.ch/id/eprint/96164

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