Identification and characterization of equine blood plasmacytoid dendritic cells.

Ziegler, Anja; Marti, Eliane Isabelle; Summerfield, Artur; Baumann, Arnaud (2016). Identification and characterization of equine blood plasmacytoid dendritic cells. Developmental and Comparative Immunology, 65, pp. 352-357. Elsevier 10.1016/j.dci.2016.08.005

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Dendritic cells (DC) are antigen-presenting cells that can be classified into three major cell subsets: conventional DC1 (cDC1), cDC2 and plasmacytoid DCs (pDC), none of which have been identified in horses. Therefore, the objective of this study was to identify and characterize DC subsets in equine peripheral blood, emphasizing on pDC. Surface marker analysis allowed distinction of putative DC subsets, according to their differential expression of CADM-1 and MHC class II. Equine pDC were found to be Flt3(+) CD4(low) CD13(-) CD14(-) CD172a(-) CADM-1(-) MHCII(low). The weak expression of CD4 on equine pDC contrasts with findings in several other mammals. Furthermore, pDC purified by fluorescence-activated cell sorting were found to be the only cell subset able to produce large amounts of IFN-α upon TLR9-agonist stimulation. The pDC identity was confirmed by demonstrating high-levels of PLAC8, RUNX2 and TCF4 expression, showing pDC-restricted expression in other mammals.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Experimental Clinical Research
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH)

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Ziegler, Anja; Marti, Eliane Isabelle; Summerfield, Artur and Baumann, Arnaud

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 630 Agriculture

ISSN:

0145-305X

Publisher:

Elsevier

Language:

English

Submitter:

Achim Braun Parham

Date Deposited:

24 Apr 2017 10:58

Last Modified:

01 Jan 2018 02:35

Publisher DOI:

10.1016/j.dci.2016.08.005

PubMed ID:

27524460

Uncontrolled Keywords:

Dendritic cell subset; Horse; Plasmacytoid dendritic cell

BORIS DOI:

10.7892/boris.96168

URI:

https://boris.unibe.ch/id/eprint/96168

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