Vig, M; Peinelt, Christine; Beck, A; Koomoa, D L; Rabah, D; Koblan-Huberson, M; Kraft, S; Turner, H; Fleig, A; Penner, R; Kinet, J-P (2006). CRACM1 is a plasma membrane protein essential for store-operated Ca2+ entry. Science, 312(5777), pp. 1220-1223. American Association for the Advancement of Science 10.1126/science.1127883
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Store-operated Ca2+ entry is mediated by Ca2+ release-activated Ca2+ (CRAC) channels following Ca2+ release from intracellular stores. We performed a genome-wide RNA interference (RNAi) screen in Drosophila cells to identify proteins that inhibit store-operated Ca2+ influx. A secondary patch-clamp screen identified CRACM1 and CRACM2 (CRAC modulators 1 and 2) as modulators of Drosophila CRAC currents. We characterized the human ortholog of CRACM1, a plasma membrane-resident protein encoded by gene FLJ14466. Although overexpression of CRACM1 did not affect CRAC currents, RNAi-mediated knockdown disrupted its activation. CRACM1 could be the CRAC channel itself, a subunit of it, or a component of the CRAC signaling machinery.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Biochemistry and Molecular Medicine |
UniBE Contributor: |
Peinelt, Christine |
Subjects: |
500 Science > 570 Life sciences; biology 600 Technology > 610 Medicine & health |
ISSN: |
0036-8075 |
Publisher: |
American Association for the Advancement of Science |
Language: |
English |
Submitter: |
Christine Peinelt |
Date Deposited: |
18 Jun 2018 10:43 |
Last Modified: |
05 Dec 2022 15:03 |
Publisher DOI: |
10.1126/science.1127883 |
PubMed ID: |
16645049 |
BORIS DOI: |
10.7892/boris.97467 |
URI: |
https://boris.unibe.ch/id/eprint/97467 |
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