Pabp binds to the osk 3'UTR and specifically contributes to osk mRNA stability and oocyte accumulation

Vazquez-Pianzola, Paula; Urlaub, Henning; Suter, Beat (2011). Pabp binds to the osk 3'UTR and specifically contributes to osk mRNA stability and oocyte accumulation. Developmental biology, 357(2), pp. 404-418. San Diego, Calif.: Elsevier 10.1016/j.ydbio.2011.07.009

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RNA localization is tightly coordinated with RNA stability and translation control. Bicaudal-D (Bic-D), Egalitarian (Egl), microtubules and their motors are part of a Drosophila transport machinery that localizes mRNAs to specific cellular regions during oogenesis and embryogenesis. We identified the Poly(A)-binding protein (Pabp) as a protein that forms an RNA-dependent complex with Bic-D in embryos and ovaries. pabp also interacts genetically with Bic-D and, similar to Bic-D, pabp is essential in the germline for oocyte growth and accumulation of osk mRNA in the oocyte. In the absence of pabp, reduced stability of osk mRNA and possibly also defects in osk mRNA transport prevent normal oocyte localization of osk mRNA. pabp also interacts genetically with osk and lack of one copy of pabp(+) causes osk to become haploinsufficient. Moreover, pointing to a poly(A)-independent role, Pabp binds to A-rich sequences (ARS) in the osk 3'UTR and these turned out to be required in vivo for osk function during early oogenesis. This effect of pabp on osk mRNA is specific for this RNA and other tested mRNAs localizing to the oocyte are less and more indirectly affected by the lack of pabp

Item Type:	Journal Article (Original Article)
Division/Institute:	08 Faculty of Science > Department of Biology > Institute of Cell Biology
UniBE Contributor:	Vazquez Pianzola, Maria Paula, Suter, Beat (A)
ISSN:	0012-1606
Publisher:	Elsevier
Language:	English
Submitter:	Factscience Import
Date Deposited:	04 Oct 2013 14:27
Last Modified:	29 Mar 2023 23:32
Publisher DOI:	10.1016/j.ydbio.2011.07.009
Web of Science ID:	000294834400012
URI:	https://boris.unibe.ch/id/eprint/10095 (FactScience: 215934)