Stoop, Britta; Defaux, Priscille Merciani; Utz, Silvia; Zieger, Martin (2017). Touch DNA sampling with SceneSafe Fast™ minitapes. Legal medicine, 29, pp. 68-71. Elsevier 10.1016/j.legalmed.2017.10.006
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To achieve optimal results in the forensic analysis of trace DNA, choosing the right collection technique is crucial. Three common approaches are currently well-established for DNA retrieval from items of clothing, notably cutting, swabbing and tape-lifting. The latter two are non-destructive and therefore preferable on items of value. Even though the most recently established technique of DNA retrieval by adhesive tapes is widely used since quite some years now, little information has been published so far on how well it performs compared to other methods. Even more important, when it comes to choosing the right DNA extraction method for forensic lifting-tapes, the available information one can rely on as a forensic geneticist is quite scarce. In our study we compared the two widely used, commercially available and automation suitable magnetic bead-based extraction methods "iPrep Forensic Kit" and "PrepFiler Express BTA™ Kit" to conventional organic solvent extraction. The results demonstrate that DNA extraction from standardized saliva samples applied to SceneSafe Fast™ minitapes is most efficient with phenol-chloroform. We also provide evidence that SceneSafe Fast™ minitapes perform better than wet cotton swabs in the sampling of touch DNA from cotton fabric. Applying the tape only once in every spot on the tissue is thereby sufficient for a considerably better collection performance of the tapes compared to swabbing.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Service Sector > Institute of Legal Medicine 04 Faculty of Medicine > Service Sector > Institute of Legal Medicine > Forensic Molecular Biology |
UniBE Contributor: |
Stoop, Britta, Utz, Silvia, Zieger, Martin |
ISSN: |
1344-6223 |
Publisher: |
Elsevier |
Language: |
English |
Submitter: |
Antoinette Angehrn |
Date Deposited: |
14 Dec 2017 08:27 |
Last Modified: |
05 Dec 2022 15:08 |
Publisher DOI: |
10.1016/j.legalmed.2017.10.006 |
PubMed ID: |
29102833 |
Uncontrolled Keywords: |
Clothing DNA extraction Sampling Tape Trace DNA |
BORIS DOI: |
10.7892/boris.107338 |
URI: |
https://boris.unibe.ch/id/eprint/107338 |
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