Thankamony, Ajay; Kemp, Graham J; Koulman, Albert; Bokii, Vlada; Savage, David B; Boesch, Chris; Hodson, Leanne; Dunger, David B; Sleigh, Alison (2018). Compositional marker in vivo reveals intramyocellular lipid turnover during fasting-induced lipolysis. Scientific Reports, 8(1), p. 2750. Nature Publishing Group 10.1038/s41598-018-21170-x
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Intramyocellular lipid (IMCL) is of particular metabolic interest, but despite many proton magnetic resonance spectroscopy (H MRS) studies reporting IMCL content measured by the methylene (CH) resonance signal, little is known about its composition. Here we validated IMCL CH:CHratio as a compositional marker usingH MRS at short echo time, and investigated IMCL content and composition during a 28-hour fast in 24 healthy males. Increases in IMCL CHrelative to the creatine and phosphocreatine resonance (Cr) at 3.0 ppm (an internal standard) correlated with circulating free fatty acid (FA) concentrations, supporting the concept of increased FA influx into IMCL. Significant decreases in IMCL CH:CHratio indicated a less unsaturated IMCL pool after fasting, and this compositional change related inversely to IMCL baseline composition, suggesting a selective efflux of unsaturated shorter-chain FA from the IMCL pool. This novel in vivo evidence reveals IMCL turnover during extended fasting, consistent with the concept of a flexible, responsive myocellular lipid store. There were also differences between soleus and tibialis anterior in basal IMCL composition and in response to fasting. We discuss the potential of this marker for providing insights into normal physiology and mechanisms of disease.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Department of Radiology, Neuroradiology and Nuclear Medicine (DRNN) > Institute of Diagnostic, Interventional and Paediatric Radiology > DCR Magnetic Resonance Spectroscopy and Methodology (AMSM) 04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Pavillon 52 > Abt. Magnetresonanz-Spektroskopie und Methodologie, AMSM |
UniBE Contributor: |
Boesch, Christoph Hans |
ISSN: |
2045-2322 |
Publisher: |
Nature Publishing Group |
Language: |
English |
Submitter: |
Christoph Hans Boesch |
Date Deposited: |
12 Apr 2018 15:18 |
Last Modified: |
02 Mar 2023 23:30 |
Publisher DOI: |
10.1038/s41598-018-21170-x |
PubMed ID: |
29426847 |
BORIS DOI: |
10.7892/boris.111818 |
URI: |
https://boris.unibe.ch/id/eprint/111818 |
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