Who needs the hotspot? The effect of temperature on the fish host immune response to Tetracapsuloides bryosalmonae the causative agent of proliferative kidney disease.

Bailey, Christyn John; Segner, Helmut; Casanova, Ayako; Wahli, Thomas (2017). Who needs the hotspot? The effect of temperature on the fish host immune response to Tetracapsuloides bryosalmonae the causative agent of proliferative kidney disease. Fish & shellfish immunology, 63, pp. 424-437. Elsevier Science 10.1016/j.fsi.2017.02.039

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Proliferative kidney disease (PKD) of salmonids, caused by Tetracapsuloides bryosalmonae may lead to high mortalities at elevated water temperatures. However, it has not yet been investigated how temperature affects the fish host immune response to T. bryosalmonae. We exposed YOY (young of the year) rainbow trout (Oncorhynchus mykiss) to T. bryosalmonae at two temperatures (12 °C and 15 °C) that reflect a realistic environmental scenario and could occur in the natural habitat of salmonids. We followed the development of the parasite, host pathology and immune response over seven weeks. We evaluated the composition and kinetics of the leukocytes and their major subgroups in the anterior and posterior kidney. We measured immune gene expression profiles associated with cell lineages and functional pathways in the anterior and posterior kidney. At 12 °C, both infection prevalence and pathogen load were markedly lower. While the immune response was characterized by subtle changes, mainly an increased amount of lymphocytes present in the kidney, elevated expression of Th1-like signature cytokines and strong upregulation of the natural killer cell enhancement factor, NKEF at week 6 P.E. At 15 °C the infection prevalence and pathogen burden were ominously greater. While the immune response as the disease progressed was associated with a Th2-like switch at week 6 P.E and a prominent B cell response, evidenced at the tissue, cell and transcript level. Our results highlight how a subtle, environmentally relevant difference in temperature resulted in diverse outcomes in terms of the immune response strategy, altering the type of interaction between a host and a parasite.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute for Fish and Wildlife Health (FIWI)

UniBE Contributor:

Bailey, Christyn John, Segner, Helmut, Casanova, Ayako, Wahli, Thomas

Subjects:

600 Technology > 630 Agriculture

ISSN:

1050-4648

Publisher:

Elsevier Science

Language:

English

Submitter:

Lucia Gugger-Raaflaub

Date Deposited:

08 May 2018 11:44

Last Modified:

05 Dec 2022 15:11

Publisher DOI:

10.1016/j.fsi.2017.02.039

PubMed ID:

28238860

Uncontrolled Keywords:

Immune response PKD Proliferative kidney disease Rainbow trout Resistance Temperature Tetracapsuloides bryosalmonae Th1 Th2 Tolerance

BORIS DOI:

10.7892/boris.112091

URI:

https://boris.unibe.ch/id/eprint/112091

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