Chevallier, Bruno; Dugourd, Dominique; Tarasiuk, Kazimirez; Harel, Josée; Gottschalk, Marcelo; Kobisch, Marylène; Frey, Joachim (1998). Chromosome sizes and phylogenetic relationships between serotypes of Actinobacillus pleuropneumoniae. FEMS microbiology letters, 160(2), pp. 209-216. Elsevier 10.1111/j.1574-6968.1998.tb12913.x
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The genome size of Actinobacillus pleuropneumoniae was determined by pulsed field gel electrophoresis of AscI and ApaI digested chromosomal DNA. The genome size of the type strain 4074T (serotype 1) was determined to be 2404±40 kb. The chromosome sizes for the reference strains of the other serotypes range between 2.3 and 2.4 Mb. The restriction pattern profiles of AscI, ApaI and NheI digested chromosomes showed a high degree of polymorphism among the different serotype reference strains and allowed their discrimination. The analysis of the macrorestriction pattern polymorphism revealed phylogenetic relationships between the different serotype reference strains which reflect to some extent groups of serotypes known to cross-react serologically. In addition, different pulsed fields gel electrophoresis patterns also revealed heterogeneity in the chromosomal structure among different field strains of serotypes 1, 5a, and 5b, while strains of serotype 9 originating from most distant geographical places showed homogeneous ApaI patterns in pulsed field gel electrophoresis.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology |
UniBE Contributor: |
Frey, Joachim |
Subjects: |
600 Technology > 630 Agriculture |
ISSN: |
0378-1097 |
Publisher: |
Elsevier |
Language: |
English |
Submitter: |
Marceline Brodmann |
Date Deposited: |
12 Nov 2020 16:09 |
Last Modified: |
05 Dec 2022 15:13 |
Publisher DOI: |
10.1111/j.1574-6968.1998.tb12913.x |
BORIS DOI: |
10.7892/boris.115532 |
URI: |
https://boris.unibe.ch/id/eprint/115532 |
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