Feulner, Philine; Schwarzer, Julia; Häsler, Marcel; Meier, Joana; Seehausen, Ole (2018). A Dense Linkage Map of Lake Victoria Cichlids Improved the Pundamilia Genome Assembly and Revealed a Major QTL for Sex-Determination. G3 Genes Genomes Genetics, 8(7), pp. 2411-2420. Genetics Society of America 10.1534/g3.118.200207
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Feulner et al. 2018_GGG_cichlid linkage map.pdf - Published Version Available under License Creative Commons: Attribution (CC-BY). Download (1MB) | Preview |
Genetic linkage maps are essential for comparative genomics, high quality genome sequence assembly and fine scale quantitative trait locus (QTL) mapping. In the present study we identified and genotyped markers via restriction-site associated DNA (RAD) sequencing and constructed a genetic linkage map based on 1,597 SNP markers of an interspecific F2 cross of two closely related Lake Victoria cichlids (Pundamilia pundamilia and P. sp. ‘red head’). The SNP markers were distributed on 22 linkage groups and
the total map size was 1,594 cM with an average marker distance of 1.01 cM. This high-resolution genetic linkage map was used to anchor the scaffolds of the Pundamilia genome and estimate recombination rates along the genome. Via QTL mapping we identified a major QTL for sex in a ~1.9 Mb region on Pun-LG10, which is homologous to Oreochromis niloticus LG 23 (Ore-LG23) and includes a well-known vertebrate sexdetermination gene (amh).
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
08 Faculty of Science > Department of Biology > Institute of Ecology and Evolution (IEE) 08 Faculty of Science > Department of Biology > Institute of Ecology and Evolution (IEE) > Aquatic Ecology |
UniBE Contributor: |
Feulner, Philine, Schwarzer, Julia, Häsler, Marcel, Meier, Joana, Seehausen, Ole |
Subjects: |
500 Science > 570 Life sciences; biology |
ISSN: |
2160-1836 |
Publisher: |
Genetics Society of America |
Language: |
English |
Submitter: |
Marcel Häsler |
Date Deposited: |
04 Jun 2018 13:47 |
Last Modified: |
05 Dec 2022 15:14 |
Publisher DOI: |
10.1534/g3.118.200207 |
PubMed ID: |
29760203 |
BORIS DOI: |
10.7892/boris.116922 |
URI: |
https://boris.unibe.ch/id/eprint/116922 |
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