Gottstein, Bruno; D'Alessandro, A; Rausch, R L (1995). Immunodiagnosis of polycystic hydatid disease/polycystic echinococcosis due to Echinococcus vogeli. The American Journal of Tropical Medicine and Hygiene, 53(5), pp. 558-563. American Society of Tropical Medicine and Hygiene 10.4269/ajtmh.1995.53.558
Full text not available from this repository. (Request a copy)A crude antigenic metacestode extract from Echinococcus vogeli was assessed by enzyme-linked immunosorbent assay (ELISA) and showed strong binding activity with serum antibodies from patients with polycystic echinococcosis. Major cross-reactions occurred with serum antibodies from patients with cystic and alveolar echinococcosis and from patients infected with other species of helminths. An E. vogeli antigen fraction, Ev2, was subsequently purified by immunosorption. The respective Ev2 ELISA demonstrated improved specificity, allowing discrimination of non-Echinococcus infections from polycystic echinococcosis. Based upon the calculation of a comparative (Ev-crude ELISA versus Ev2 ELISA) reactivity index, it became possible to discriminate all cystic echinococcosis cases, but only some alveolar echinococcosis cases, from polycystic echinococcosis. Immunoblot analyses revealed an antibody banding pattern highly conserved among polycystic, cystic, and alveolar echinococcosis. However, immunoblotting reliably distinguished between echinococcosis and all non-Echinococcus infections.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Parasitology 05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) |
UniBE Contributor: |
Gottstein, Bruno |
Subjects: |
600 Technology > 610 Medicine & health 600 Technology > 630 Agriculture |
ISSN: |
0002-9637 |
Publisher: |
American Society of Tropical Medicine and Hygiene |
Language: |
English |
Submitter: |
Bruno Gottstein |
Date Deposited: |
18 Jul 2018 15:06 |
Last Modified: |
05 Dec 2022 15:16 |
Publisher DOI: |
10.4269/ajtmh.1995.53.558 |
PubMed ID: |
7485718 |
URI: |
https://boris.unibe.ch/id/eprint/118695 |
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