Hemphill, A; Fuchs, N; Sonda, S; Gottstein, Bruno; Hentrich, B (1997). Identification and partial characterization of a 36 kDa surface protein on Neospora caninum tachyzoites. Parasitology, 115(04), pp. 371-380. Cambridge University Press 10.1017/S0031182097001455
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identification-and-partial-characterization-of-a-36-kda-surface-protein-on-neospora-caninum-tachyzoites.pdf - Published Version Available under License Publisher holds Copyright. Download (700kB) | Preview |
Neospora caninum, the causative agent of neosporosis, is a recently identified apicomplexan parasite which is structurally and biologically closely related to, but antigenically distinct from, Toxoplasma gondii. Molecules associated with the surfaces of N. caninum tachyzoites are likely to participate in the host cell entry process, could be involved in the interaction of the parasite with the immune system, and they could influence the pathogenesis of neosporosis. Isolated N. caninum tachyzoites were extracted with the non-ionic detergent Triton X-114 and were further analysed using a polyclonal anti-N. caninum antiserum. Immunoblots revealed several reactive bands, 1 of which represented a glycoprotein of approximately 36 kDa (Nc-p36). This molecule was present in 2 isolates of Neospora (NC-1 and Liverpool), but was absent in Toxoplasma (RH-strain) tachyzoites. Immunofluorescence and pre-embedding immunogold transmission electron microscopy employing affinity-purified anti-Nc-p36 antibodies showed that the Nc-p36 is a cell surface-associated protein. Immunogold on-section labelling of LR-White-embedded parasites, fixed prior and at defined time-points after host cell entry, demonstrated the presence of this molecule on the surface as well as within the dense granules of N. caninum tachyzoites.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Parasitology |
UniBE Contributor: |
Gottstein, Bruno |
Subjects: |
600 Technology > 610 Medicine & health 600 Technology > 630 Agriculture |
ISSN: |
0031-1820 |
Publisher: |
Cambridge University Press |
Language: |
English |
Submitter: |
Bruno Gottstein |
Date Deposited: |
19 Jul 2018 15:35 |
Last Modified: |
05 Dec 2022 15:16 |
Publisher DOI: |
10.1017/S0031182097001455 |
PubMed ID: |
9364563 |
BORIS DOI: |
10.48350/118724 |
URI: |
https://boris.unibe.ch/id/eprint/118724 |
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