Ingold, K; Gottstein, Bruno; Hemphill, A (1998). Identification of a laminated layer-associated protein in Echinococcus multilocularis metacestodes. Parasitology, 116(04), pp. 363-372. Cambridge University Press 10.1017/S0031182098002406
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identification-of-a-laminated-layer-associated-protein-in-echinococcus-multilocularis-metacestodes.pdf - Published Version Available under License Publisher holds Copyright. Download (395kB) | Preview |
Echinococcus multilocularis is a cestode parasite that predominantly infects red and arctic foxes as definitive hosts. Ingestion of E. multilocularis eggs and subsequent post-oncospheral infection with the larval stage (metacestode) of the parasite results in alveolar echinococcosis (AE), a life-threatening hepatic disease concerning humans and other intermediate hosts such as small rodents. The primary fluid-filled vesicles of the asexually proliferating metacestode are comprised of an inner germinal layer, a syncytial tegument, and an outer, acellular, so-called laminated layer. This laminated layer may play an important role in protecting the developing E. multilocularis metacestode from host immune reactions, and laminated layer-associated components represent potential targets for intervention during the course of AE. We have used an in vitro cultivation technique for the long-term maintenance and proliferation of E. multilocularis metacestodes in order to generate premature (protoscolex-free) parasite vesicles. A polyclonal antiserum was raised against this host-free parasite tissue. Subsequent immunoblot analysis of parasite fractions obtained by Triton X-114 extraction lead to the identification of a 116 kDa protein (named EmP2) within the Triton-insoluble fraction. The characterization of EmP2 by SDS-PAGE, Western blotting, and by immunofluorescence revealed that EmP2 is a laminated layer-associated protein.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Parasitology |
UniBE Contributor: |
Gottstein, Bruno |
Subjects: |
600 Technology > 630 Agriculture |
ISSN: |
0031-1820 |
Publisher: |
Cambridge University Press |
Language: |
English |
Submitter: |
Bruno Gottstein |
Date Deposited: |
19 Jul 2018 15:39 |
Last Modified: |
05 Dec 2022 15:16 |
Publisher DOI: |
10.1017/S0031182098002406 |
PubMed ID: |
9585938 |
BORIS DOI: |
10.48350/118734 |
URI: |
https://boris.unibe.ch/id/eprint/118734 |
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