Abril, C.; Thomann, Andreas; Brodard, Isabelle; Wu, Natacha; Ryser-Degiorgis, Marie-Pierre; Frey, Joachim; Overesch, Gudrun (2011). A novel isolation method of Brucella species and molecular tracking of Brucella suis biovar 2 in domestic and wild animals. Veterinary microbiology, 150(3-4), pp. 405-10. Amsterdam: Elsevier 10.1016/j.vetmic.2011.02.056
Full text not available from this repository. (Request a copy)Brucella suis biovar 2 is the most common aetiological agent of porcine brucellosis in Europe. B. suis biovar 2 is considered to have low zoonotic potential, but is a causative agent of reproductive losses in pigs, and it is thus economically important. The multilocus variable-number of tandem repeats genotyping analysis of 16 loci (MLVA-16) has proven to be highly discriminatory and is the most suitable assay for simultaneously identifying B. suis and tracking infections. The aim of this study was to investigate the relatedness between isolates of B. suis biovar 2 obtained during a brucellosis outbreak in domestic pigs and isolates from wild boars and hares collected from proximal or remote geographical areas by MLVA-16. A cluster analysis of the MLVA-16 data revealed that most of the isolates obtained from Switzerland clustered together, with the exception of one isolate. The outbreak isolates constituted a unique subcluster (with a genetic similarity >93.8%) distinct from that of the isolates obtained from wild animals, suggesting that direct transmission of the bacterium from wild boars to domestic pigs did not occur in this outbreak. To obtain a representative number of isolates for MLVA-16, alternative methods of Brucella spp. isolation from tissue samples were compared with conventional direct cultivation on a Brucella-selective agar. We observed an enhanced sensitivity when mechanical homogenisation was followed by host cell lysis prior to cultivation on the Brucella-selective agar. This work demonstrates that MLVA-16 is an excellent tool for both monitoring brucellosis and investigating outbreaks. Additionally, we present efficient alternatives for the isolation of Brucella spp.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology 05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Center for Fish and Wildlife Health (FIWI) 05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) |
UniBE Contributor: |
Abril Gaona, Carlos, Thomann, Andreas, Brodard, Isabelle, Wu, Natacha, Ryser, Marie Pierre, Frey, Joachim, Overesch, Gudrun |
Subjects: |
600 Technology > 630 Agriculture |
ISSN: |
0378-1135 |
Publisher: |
Elsevier |
Language: |
English |
Submitter: |
Factscience Import |
Date Deposited: |
04 Oct 2013 14:31 |
Last Modified: |
05 Dec 2022 14:09 |
Publisher DOI: |
10.1016/j.vetmic.2011.02.056 |
URI: |
https://boris.unibe.ch/id/eprint/11969 (FactScience: 218229) |
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