Update on Tetracycline Susceptibility of Pediococcus acidilactici Based on Strains Isolated from Swiss Cheese and Whey.

Lüdin, Petra; Roetschi, Alexandra; Wüthrich, Daniel; Bruggmann, Rémy; Berthoud, Hélène; Shani, Noam (2018). Update on Tetracycline Susceptibility of Pediococcus acidilactici Based on Strains Isolated from Swiss Cheese and Whey. Journal of food protection, 81(10), pp. 1582-1589. International Association for Food Protection 10.4315/0362-028X.JFP-18-160

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Bacterial strains used as starter cultures in the production of fermented foods may act as reservoirs for antibiotic resistance (AbR) genes. To avoid the introduction of such genes into the food chain, the presence of acquired AbR in bacterial strains added to food must be tested. Standard protocols and microbiological cut-off values have been defined to provide practitioners with a basis for evaluating whether their bacterial isolates harbor an acquired resistance to a given antibiotic. Here, we tested the AbR of 24 strains of Pediococcus acidilactici by using the standard protocol and microbiological cut-off values recommended by the European Food Safety Authority. Phenotypic data were complemented by searching for known AbR genes using an in silico analysis of whole genomes. The majority (54.2%) of the strains were able to grow at a tetracycline concentration above the defined cut-off, even though only one strain carried a known tetracycline resistance gene, tetM. The same strain also carried the AbR gene of an erythromycin resistance methylase, ermA, and displayed resistance toward clindamycin and erythromycin. Our results bolster the scarce data on the sensitivity of P. acidilactici to tetracycline and suggest that the microbiological cut-off recommended by the European Food Safety Authority for this antibiotic should be amended.

Item Type:	Journal Article (Original Article)
Division/Institute:	08 Faculty of Science > Department of Biology > Bioinformatics and Computational Biology > Bioinformatics 08 Faculty of Science > Department of Biology > Bioinformatics and Computational Biology > Computational Biology 08 Faculty of Science > Department of Biology > Bioinformatics and Computational Biology
UniBE Contributor:	Wüthrich, Daniel, Bruggmann, Rémy
ISSN:	0362-028X
Publisher:	International Association for Food Protection
Language:	English
Submitter:	Jolanda Paganoni Zurbrügg
Date Deposited:	03 Jan 2020 08:53
Last Modified:	04 May 2023 12:50
Publisher DOI:	10.4315/0362-028X.JFP-18-160
PubMed ID:	30169118
Uncontrolled Keywords:	Anti-Bacterial Agents,Cheese,Drug Resistance, Bacterial,Microbial Sensitivity Tests,Pediococcus,Pediococcus acidilactici,Tetracycline,Whey,drug effects,microbiology,pharmacology
URI:	https://boris.unibe.ch/id/eprint/136640