Absolute Quantification of Noncoding RNA by Microscale Thermophoresis

Jacob, Dominik; Thüring, Kathrin; Galliot, Aurellia; Marchand, Virginie; Galvanin, Adeline; Ciftci, Akif; Scharmann, Karin; Stock, Michael; Roignant, Jean-Yves; Leidel, Sebastian Andreas; Motorin, Yuri; Schaffrath, Raffael; Klassen, Roland; Helm, Mark (2019). Absolute Quantification of Noncoding RNA by Microscale Thermophoresis. Angewandte Chemie (International ed.), 58(28), pp. 9565-9569. Wiley-VCH 10.1002/anie.201814377

Preview

Text Jacob_et_al-2019-Angewandte_Chemie_International_Edition.pdf - Published Version Available under License Creative Commons: Attribution-Noncommercial (CC-BY-NC). Download (2MB) | Preview

Accurate quantification of the copy numbers of noncoding RNA has recently emerged as an urgent problem, with impact on fields such as RNA modification research, tissue differentiation, and others. Herein, we present a hybridization-based approach that uses microscale thermophoresis (MST) as a very fast and highly precise readout to quantify, for example, single tRNA species with a turnaround time of about one hour. We developed MST to quantify the effect of tRNA toxins and of heat stress and RNA modification on single tRNA species. A comparative analysis also revealed significant differences to RNA-Seq-based quantification approaches, strongly suggesting a bias due to tRNA modifications in the latter. Further applications include the quantification of rRNA as well as of polyA levels in cellular RNA.

Interest & Impact

Downloads

247 since deposited on 07 Jan 2020
22 in the past 12 months

Citations

5 Citations in Web of Science ®
6 Citations in Scopus

Search

in Google Scholar™

Services

Actions (login required)

Edit item

Actions (login required)

Edit item