Bosshard, P. P.; Kronenberg, A.; Zbinden, R.; Ruef, C.; Bottger, E. C.; Altwegg, M. (2003). Etiologic Diagnosis of Infective Endocarditis by Broad-Range Polymerase Chain Reaction: A 3-Year Experience. Clinical infectious diseases, 37(2), pp. 167-172. The University of Chicago Press 10.1086/375592
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We analyzed surgically resected endocardial specimens from 49 patients by broad-range PCR. PCR results were compared with (1) results of previous blood cultures, (2) results of culture and Gram staining of resected specimens, and (3) clinical data (Duke criteria). Molecular analyses of resected specimens and previous blood cultures showed good overall agreement. However, in 18% of patients with sterile blood cultures, bacterial DNA was found in the resected materials. When data from patients with definite or rejected cases of infective endocarditis (IE) were included, the sensitivity, specificity, and positive and negative predictive values of broad-range PCR were 82.6%, 100%, 100%, and 76.5%, respectively, overall, and 94.1%, 100%, 100%, and 90%, for cases of native valve endocarditis. The sensitivity, specificity, and positive and negative predictive values of culture of resected specimens from patients with native valve endocarditis were 17.6%, 88.9%, 75%, and 36.4%. We recommend broad-range PCR of surgically resected endocardial material in cases of possible IE, in cases of suspected IE in which blood cultures are sterile, and in cases in which organisms grow in blood cultures but only Duke minor criteria are met. We propose to add molecular techniques to the pathologic criteria of the Duke classification scheme.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Clinic of Infectiology |
UniBE Contributor: |
Kronenberg, Andreas Oskar |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
1058-4838 |
Publisher: |
The University of Chicago Press |
Language: |
English |
Submitter: |
Andreas Oskar Kronenberg |
Date Deposited: |
03 Feb 2021 10:48 |
Last Modified: |
05 Dec 2022 15:36 |
Publisher DOI: |
10.1086/375592 |
PubMed ID: |
12856207 |
BORIS DOI: |
10.48350/139171 |
URI: |
https://boris.unibe.ch/id/eprint/139171 |
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