Self-Assembly of α-Tocopherol Transfer Protein Nanoparticles: A Patchy Protein Model.

Peltzer, Raphael Mathias; Kolli, Hima Bindu; Stocker, Achim; Cascella, Michele (2018). Self-Assembly of α-Tocopherol Transfer Protein Nanoparticles: A Patchy Protein Model. The Journal of Physical Chemistry B, 122(28), pp. 7066-7072. American Chemical Society 10.1021/acs.jpcb.8b05936

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We describe the mechanism of self-aggregation of α-tocopherol transfer protein into a spherical nanocage employing Monte Carlo simulations. The protein is modeled by a patchy coarse-grained representation, where the protein-protein interfaces, determined in the past by X-ray diffraction, are represented by simplified two-body interaction potentials. Our results show that the oligomerization kinetics proceeds in two steps, with the formation of metastable trimeric units and the subsequent assembly into the spherical aggregates. Data are in agreement with experimental observations regarding the prevalence of different aggregation states at specific ambient conditions. Finally, our results indicate a route for the experimental stabilization of the trimer, crucial for the understanding of the physiological role of such aggregates in vitamin E body trafficking.

Item Type:	Journal Article (Original Article)
Division/Institute:	08 Faculty of Science > Department of Chemistry, Biochemistry and Pharmaceutical Sciences (DCBP)
UniBE Contributor:	Stocker, Achim
Subjects:	500 Science > 570 Life sciences; biology 500 Science > 540 Chemistry
ISSN:	1520-5207
Publisher:	American Chemical Society
Language:	English
Submitter:	Anja Ebeling
Date Deposited:	11 Mar 2020 15:21
Last Modified:	05 Dec 2022 15:37
Publisher DOI:	10.1021/acs.jpcb.8b05936
PubMed ID:	29944374
URI:	https://boris.unibe.ch/id/eprint/141794