Kargarpour, Zahra; Nasirzade, Jila; Panahipour, Layla; Miron, Richard J.; Gruber, Reinhard (2020). Relative Centrifugal Force (RCF; G-Force) Affects the Distribution of TGF-β in PRF Membranes Produced Using Horizontal Centrifugation. International journal of molecular sciences, 21(20) Molecular Diversity Preservation International MDPI 10.3390/ijms21207629
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Solid platelet-rich fibrin (PRF) is produced with centrifugation tubes designed to accelerate clotting. Thus, activated platelets may accumulate within the fibrin-rich extracellular matrix even before centrifugation is initiated. It can thus be assumed that platelets and their growth factors such as transforming growth factor-β (TGF-β) are trapped within PRF independent of their relative centrifugal force (RCF), the gravitation or g-force. To test this assumption, we prepared PRF membranes with tubes where clotting is activated by a silicone-coated interior. Tubes underwent 210 g, 650 g and 1500 g for 12 min in a horizontal centrifuge. The respective PRF membranes, either in total or separated into a platelet-poor plasma and buffy coat fraction, were subjected to repeated freeze-thawing to prepare lysates. Gingival fibroblasts were exposed to the PRF lysates to provoke the expression of TGF-β target genes. We show here that the expression of interleukin 11 (IL11) and NADPH oxidase 4 (NOX4), and Smad2/3 signaling were similarly activated by all lysates when normalized to the size of the PRF membranes. Notably, platelet-poor plasma had significantly less TGF-β activity than the buffy coat fraction at both high-speed protocols. In contrast to our original assumption, the TGF-β activity in PRF lysates produced using horizontal centrifugation follows a gradient with increasing concentration from the platelet-poor plasma towards the buffy coat layer.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology 04 Faculty of Medicine > School of Dental Medicine > Periodontics Research |
UniBE Contributor: |
Miron, Richard John, Gruber, Reinhard |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
1661-6596 |
Publisher: |
Molecular Diversity Preservation International MDPI |
Language: |
English |
Submitter: |
Doris Burri |
Date Deposited: |
06 Jan 2021 11:49 |
Last Modified: |
05 Dec 2022 15:43 |
Publisher DOI: |
10.3390/ijms21207629 |
PubMed ID: |
33076376 |
Uncontrolled Keywords: |
TGF-β buffy coat fibrin gravitational force platelet-poor plasma platelet-rich fibrin |
BORIS DOI: |
10.48350/149749 |
URI: |
https://boris.unibe.ch/id/eprint/149749 |
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