Lack of an exaggerated inflammatory response on virus infection in cystic fibrosis

Kieninger, E; Vareille, M; Kopf, BS; Blank, F; Alves, MP; Gisler, FM; Latzin, P; Casaulta, C; Geiser, T; Johnston, SL; Edwards, MR; Regamey, N (2012). Lack of an exaggerated inflammatory response on virus infection in cystic fibrosis. European respiratory journal, 39(2), pp. 297-304. Sheffield, UK: European Respiratory Society 10.1183/09031936.00054511

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Respiratory virus infections play an important role in cystic fibrosis (CF) exacerbations, but underlying pathophysiological mechanisms are poorly understood. We aimed to assess whether an exaggerated inflammatory response of the airway epithelium on virus infection could explain the increased susceptibility of CF patients towards respiratory viruses. We used primary bronchial and nasal epithelial cells obtained from 24 healthy control subjects and 18 CF patients. IL-6, IL-8/CXCL8, IP-10/CXCL10, MCP-1/CCL2, RANTES/CCL5 and GRO-α/CXCL1 levels in supernatants and mRNA expression in cell lysates were measured before and after infection with rhinoviruses (RV-16 and RV-1B) and RSV. Cytotoxicity was assessed by lactate dehydrogenate assay and flow cytometry. All viruses induced strong cytokine release in both control and CF cells. The inflammatory response on virus infection was heterogeneous and depended on cell type and virus used, but was not increased in CF compared with control cells. On the contrary, there was a marked trend towards lower cytokine production associated with increased cell death in CF cells. An exaggerated inflammatory response to virus infection in bronchial epithelial cells does not explain the increased respiratory morbidity after virus infection in CF patients.

Item Type:	Journal Article (Original Article)
Division/Institute:	04 Faculty of Medicine > Department of Gynaecology, Paediatrics and Endocrinology (DFKE) > Clinic of Paediatric Medicine 04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Pneumology
UniBE Contributor:	Kieninger, Elisabeth, Vareille, Marjolaine, Blank, Fabian, Gisler, Franziska, Latzin, Philipp, Geiser, Thomas (A), Regamey, Nicolas
ISSN:	0903-1936
ISBN:	0903-1936
Publisher:	European Respiratory Society
Language:	English
Submitter:	Anette van Dorland
Date Deposited:	04 Oct 2013 14:37
Last Modified:	29 Mar 2023 23:32
Publisher DOI:	10.1183/09031936.00054511
PubMed ID:	21719483
Additional Information:	Kieninger, E. Vareille, M. Kopf, B. S. Blank, F. Alves, M. P. Gisler, F. M. Latzin, P. Casaulta, C. Geiser, T. Johnston, S. L. Edwards, M. R. Regamey, N.
URI:	https://boris.unibe.ch/id/eprint/15070 (FactScience: 222234)