Prolyl hydroxylase inhibitors increase the production of vascular endothelial growth factor in dental pulp-derived cells

Müller, Heinz-Dieter; Cvikl, Barbara; Gruber, Reinhard; Watzek, Georg; Agis, Hermann (2012). Prolyl hydroxylase inhibitors increase the production of vascular endothelial growth factor in dental pulp-derived cells. Journal of endodontics, 38(11), pp. 1498-1503. Hagerstown, Md.: Elsevier 10.1016/j.joen.2012.08.003

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Introduction: Prolyl hydroxylase (PHD) inhibitors can induce a proangiogenic response that stimulates regeneration in soft and hard tissues. However, the effect of PHD inhibitors on the dental pulp is unclear. The purpose of this study was to evaluate the effects of PHD inhibitors on the proangiogenic capacity of human dental pulp–derived cells.

Methods: To test the response of dental pulp–derived cells to PHD inhibitors, the cells were exposed to dimethyloxalylglycine, desferrioxamine, L-mimosine, and cobalt chloride. To assess the response of dental pulp cells to a capping material supplemented with PHD inhibitors, the cells were treated with supernatants from calcium hydroxide. Viability, proliferation, and protein synthesis were assessed by formazan formation, 3[H]thymidine, and 3[H]leucine incorporation assays. The effect on the proangiogenic capacity was measured by immunoassays for vascular endothelial growth factor (VEGF).

Results: We found that all 4 PHD inhibitors can reduce viability, proliferation, and protein synthesis at high concentrations. At nontoxic concentrations and in the presence of supernatants from calcium hydroxide, PHD inhibitors stimulated the production of VEGF in dental pulp–derived cells. When calcium hydroxide was supplemented with the PHD inhibitors, the supernatants from these preparations did not significantly elevate VEGF levels.

Conclusions: These results show that PHD inhibitors can stimulate VEGF production of dental pulp–derived cells, suggesting a corresponding increase in their proangiogenic capacity. Further studies will be required to understand the impact that this might have on pulp regeneration.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology

UniBE Contributor:

Cvikl, Barbara, Gruber, Reinhard

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0099-2399

Publisher:

Elsevier

Language:

English

Submitter:

Eveline Carmen Schuler

Date Deposited:

04 Oct 2013 14:38

Last Modified:

05 Dec 2022 14:11

Publisher DOI:

10.1016/j.joen.2012.08.003

PubMed ID:

23063224

Web of Science ID:

000310716600006

Uncontrolled Keywords:

Angiogenesis; dental pulp–derived cells; hypoxia-inducible factor 1α; prolyl hydroxylase inhibitors; pulp capping; pulp regeneration; vascular endothelial growth factor

BORIS DOI:

10.7892/boris.15165

URI:

https://boris.unibe.ch/id/eprint/15165 (FactScience: 222410)

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