Milijaš Jotić, Matej Milijaš; Panevska, Anastasija; Iacovache, Ioan; Kostanjšek, Rok; Mravinec, Martina; Skočaj, Matej; Zuber, Benoît; Pavšič, Ana; Razinger, Jaka; Modic, Špela; Trenti, Francesco; Guella, Graziano; Sepčić, Kristina (2021). Dissecting Out the Molecular Mechanism of Insecticidal Activity of Ostreolysin A6/Pleurotolysin B Complexes on Western Corn Rootworm. Toxins, 13(7) MDPI 10.3390/toxins13070455
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Ostreolysin A6 (OlyA6) is a protein produced by the oyster mushroom (Pleurotus ostreatus). It binds to membrane sphingomyelin/cholesterol domains, and together with its protein partner, pleurotolysin B (PlyB), it forms 13-meric transmembrane pore complexes. Further, OlyA6 binds 1000 times more strongly to the insect-specific membrane sphingolipid, ceramide phosphoethanolamine (CPE). In concert with PlyB, OlyA6 has potent and selective insecticidal activity against the western corn rootworm. We analysed the histological alterations of the midgut wall columnar epithelium of western corn rootworm larvae fed with OlyA6/PlyB, which showed vacuolisation of the cell cytoplasm, swelling of the apical cell surface into the gut lumen, and delamination of the basal lamina underlying the epithelium. Additionally, cryo-electron microscopy was used to explore the membrane interactions of the OlyA6/PlyB complex using lipid vesicles composed of artificial lipids containing CPE, and western corn rootworm brush border membrane vesicles. Multimeric transmembrane pores were formed in both vesicle preparations, similar to those described for sphingomyelin/cholesterol membranes. These results strongly suggest that the molecular mechanism of insecticidal action of OlyA6/PlyB arises from specific interactions of OlyA6 with CPE, and the consequent formation of transmembrane pores in the insect midgut.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
09 Interdisciplinary Units > Microscopy Imaging Center (MIC) 04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy |
UniBE Contributor: |
Iacovache, Mircea Ioan, Zuber, Benoît |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
2072-6651 |
Publisher: |
MDPI |
Language: |
English |
Submitter: |
Benoît Zuber |
Date Deposited: |
05 Jul 2021 15:32 |
Last Modified: |
05 Dec 2022 15:51 |
Publisher DOI: |
10.3390/toxins13070455 |
PubMed ID: |
34209983 |
BORIS DOI: |
10.48350/157261 |
URI: |
https://boris.unibe.ch/id/eprint/157261 |
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