Allergen extract- and component-based diagnostics in children of the ALLIANCE asthma cohort.

Skevaki, Chrysanthi; Tafo, Pavel; Eiringhaus, Kathrin; Timmesfeld, Nina; Weckmann, Markus; Happle, Christine; Nelson, Philipp P; Maison, Nicole; Schaub, Bianca; Ricklefs, Isabell; Fuchs, Oliver; von Mutius, Erika; Kopp, Matthias Volkmar; Renz, Harald; Hansen, Gesine; Dittrich, Anna-Maria (2021). Allergen extract- and component-based diagnostics in children of the ALLIANCE asthma cohort. Clinical and experimental allergy, 51(10), pp. 1331-1345. Wiley 10.1111/cea.13964

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BACKGROUND

Current in vitro allergen-specific IgE (sIgE) detection assays measure IgE against allergen extracts or molecules in a single- or multiplex approach. Direct comparisons of the performance of such assays among young children with common presentations of allergic diseases regardless of sensitization status are largely missing.

OBJECTIVES

The aim of this study was a comparison of the analytical and diagnostic performance for common clinical questions of three commonly used technologies which rely upon different laboratory methodologies among children of the All Age Asthma (ALLIANCE) cohort (clinicaltrials.gov: NCT02496468).

METHODS

Sera from 106 paediatric study participants (mean age 4 years) were assessed for the presence of sIgE by means of the ImmunoCAP™ sx1 and fx5 mixes, the ImmunoCAP ISAC™ 112 microarray and a Euroline™ panel.

RESULTS

Total and negative concordance was high (>82%->89%), while positive concordance varied considerably (0%-100%) but was also >50% for the most common sensitizations analysed (house dust mite and birch). All three test systems showed good sensitivity and specificity (AUC consistently > 0.7). However, no significant differences with regard to identifying sIgE sensitizations associated with symptoms in children with suspected pollen- or dust-triggered wheeze or presenting with symptoms of allergic rhinoconjunctivitis or food allergy were detected. Extending the number of allergens did not change the similar performance of the three assay systems.

CONCLUSION AND CLINICAL RELEVANCE

Among young children, the three sIgE assays showed good analytical and diagnostic concordance. Our results caution that the identification of larger numbers of sensitizations by more comprehensive multiplex approaches may not improve the clinical utility of sIgE testing in this age group.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Gynaecology, Paediatrics and Endocrinology (DFKE) > Clinic of Paediatric Medicine
04 Faculty of Medicine > Department of Gynaecology, Paediatrics and Endocrinology (DFKE) > Clinic of Paediatric Medicine > Paediatric Pneumology

UniBE Contributor:

Fuchs, Oliver, Kopp, Matthias Volkmar

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1365-2222

Publisher:

Wiley

Language:

English

Submitter:

Anette van Dorland

Date Deposited:

23 Aug 2021 15:44

Last Modified:

05 Dec 2022 15:52

Publisher DOI:

10.1111/cea.13964

PubMed ID:

34128558

Uncontrolled Keywords:

allergen sIgE analytical performance diagnostic performance in vitro allergy diagnosis molecular allergology

BORIS DOI:

10.48350/157924

URI:

https://boris.unibe.ch/id/eprint/157924

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