de Coulon, Etienne; Dellenbach, Christian; Rohr, Stephan (2021). Advancing mechanobiology by performing whole-cell patch clamp recording on mechanosensitive cells subjected simultaneously to dynamic stretch events. iScience, 24(2), p. 102041. Elsevier 10.1016/j.isci.2021.102041
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A comprehensive understanding of mechano-electrical coupling requires continuous intracellular electrical recordings being performed on cells undergoing simultaneously in vivo like strain events. Here, we introduce a linear strain single-cell electrophysiology (LSSE) system that meets these requirements by delivering highly reproducible unidirectional strain events with magnitudes up to 12% and strain rates exceeding 200%s-1 to adherent cells kept simultaneously in whole-cell patch-clamp recording configuration. Proof-of-concept measurements with NIH3T3 cells demonstrate that stable recording conditions are maintained over tens of strain cycles at maximal amplitudes and strain rates thereby permitting a full electrophysiological characterization of mechanically activated ion currents. Because mechano-electrical responses to predefined strain patterns can be investigated using any adherent wild-type or genetically modified cell type of interest, the LSSE system offers the perspective of providing advanced insights into mechanosensitive ion channel function that can finally be compared quantitatively among different types of channels and cells.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Physiology |
UniBE Contributor: |
de Coulon, Etienne Olivier, Dellenbach, Christian, Rohr, Stephan |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
2589-0042 |
Publisher: |
Elsevier |
Language: |
English |
Submitter: |
Stefan von Känel-Zimmermann |
Date Deposited: |
21 Oct 2021 10:32 |
Last Modified: |
05 Dec 2022 15:53 |
Publisher DOI: |
10.1016/j.isci.2021.102041 |
PubMed ID: |
33532717 |
BORIS DOI: |
10.48350/159858 |
URI: |
https://boris.unibe.ch/id/eprint/159858 |
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