Rieger, Michael; Dellenbach, Christian; vom Berg, Johannes; Beil-Wagner, Jane; Maguy, Ange; Rohr, Stephan (2021). Enabling comprehensive optogenetic studies of mouse hearts by simultaneous opto-electrical panoramic mapping and stimulation. Nature communications, 12(1), p. 5804. Nature Publishing Group 10.1038/s41467-021-26039-8
|
Text
Rieger2021Enablingco.pdf - Published Version Available under License Creative Commons: Attribution (CC-BY). Download (8MB) | Preview |
During the last decade, cardiac optogenetics has turned into an essential tool for investigating cardiac function in general and for assessing functional interactions between different myocardial cell types in particular. To advance exploitation of the unique research opportunities offered by this method, we develop a panoramic opto-electrical measurement and stimulation (POEMS) system for mouse hearts. The core of the experimental platform is composed of 294 optical fibers and 64 electrodes that form a cup which embraces the entire ventricular surface of mouse hearts and enables straightforward 'drop&go' experimentation. The flexible assignment of fibers and electrodes to recording or stimulation tasks permits a precise tailoring of experiments to the specific requirements of individual optogenetic constructs thereby avoiding spectral congestion. Validation experiments with hearts from transgenic animals expressing the optogenetic voltage reporters ASAP1 and ArcLight-Q239 demonstrate concordance of simultaneously recorded panoramic optical and electrical activation maps. The feasibility of single fiber optical stimulation is proven with hearts expressing the optogenetic voltage actuator ReaChR. Adaptation of the POEMS system to larger hearts and incorporation of additional sensors can be achieved by redesigning the system-core accordingly.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Physiology |
UniBE Contributor: |
Rieger, Michael, Dellenbach, Christian, Maguy, Ange, Rohr, Stephan |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
2041-1723 |
Publisher: |
Nature Publishing Group |
Language: |
English |
Submitter: |
Stefan von Känel-Zimmermann |
Date Deposited: |
26 Oct 2021 14:21 |
Last Modified: |
05 Dec 2022 15:53 |
Publisher DOI: |
10.1038/s41467-021-26039-8 |
PubMed ID: |
34608155 |
BORIS DOI: |
10.48350/159884 |
URI: |
https://boris.unibe.ch/id/eprint/159884 |
Actions (login required)
 |
Edit item |