Beyond Trial and Error: A Systematic Development of Liposomes Targeting Primary Macrophages

Weber, Florian; C. Ivan, Daniela; Proulx, Steven T.; Locatelli, Giuseppe; Aleandri, Simone; Luciani, Paola (2021). Beyond Trial and Error: A Systematic Development of Liposomes Targeting Primary Macrophages. Advanced nanoBiomed research, 1(3), p. 2000098. Wiley 10.1002/anbr.202000098

Preview

Text anbr.202000098.pdf - Published Version Available under License Creative Commons: Attribution (CC-BY). Download (2MB) | Preview

Monocytes/macrophages are phagocytic innate immune cells playing a pivotal role in tissue homeostasis, inflammation, and antitumor immunity in a microenvironment-dependent manner. By expressing pattern recognition and scavenger receptors on their surface, macrophages selectively take up pathogens, cellular debris, and often—undesirably—drug delivery systems. On the other hand, the propensity of phagocytic cells to internalize particulate drug carriers is used to load them with a cargo of choice, turning the monocytes/macrophages into a diagnostic or therapeutic Trojan horse. Identifying the ideal physicochemical properties of particulate carriers such as liposomes to achieve the most efficient macrophage-mediated drug delivery has been object of extensive research in the past, but the studies reported so far rely solely on trial-and-error approaches. Herein, a design of experiment (DoE) strategy to identify the optimal liposomal formulation is proposed, fully characterized in terms of size, surface charge, and membrane fluidity, to maximize macrophage targeting. The findings are validated using mouse bone marrow-derived macrophages, a primary preparation modeling in vivo monocyte-derived macrophages, thus confirming the robustness and versatility of the systematic and iterative approach and suggesting the promising potential of the DoE approach for the design of cell-targeting delivery systems.

Interest & Impact

Downloads

247 since deposited on 27 Dec 2021
47 in the past 12 months

Citations

5 Citations in Web of Science ®
6 Citations in Scopus

Search

in Google Scholar™

Services

Actions (login required)

Edit item

Actions (login required)

Edit item