Dysli, Chantal; Dysli, Muriel; Lincke, Joel; Jaggi, Damian; Wolf, Sebastian; Zinkernagel, Martin S. (2021). Imaging artifacts in fluorescence lifetime imaging ophthalmoscopy. Retina - the journal of retinal and vitreous diseases, 41(11), pp. 2378-2390. Lippincott, Williams & Wilkins 10.1097/IAE.0000000000003235
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IMAGING_ARTIFACTS_IN_FLUORESCENCE_LIFETIME_IMAGING.23.pdf - Published Version Available under License Creative Commons: Attribution-Noncommercial-No Derivative Works (CC-BY-NC-ND). Download (1MB) | Preview |
Purpose: To investigate and quantify the influence of imaging artifacts on retinal fluorescence lifetime (FLIO) values and to provide helpful hints and tricks to avoid imaging artifacts and to improve FLIO image acquisition quality.
Methods: A systematic analysis of potential parameters influencing FLIO quality and/or fluorescence lifetime values was performed in a prospective systematic experimental imaging study in five eyes of five healthy subjects. For image acquisition, a fluorescence lifetime imaging ophthalmoscope (Heidelberg Engineering) was used. Quantitative analysis of FLIO lifetime changes due to imaging artifacts was performed.
Results: Imaging artifacts with significant influence on fluorescence lifetimes included too short image acquisition time, insufficient illumination, ocular surface problems, and image defocus. Prior use of systemic or topical fluorescein makes analysis of retinal fluorescence lifetimes impossible.
Conclusion: Awareness of possible sources of imaging artifacts is important for FLIO image acquisition and analysis. Therefore, standardized imaging and analysis procedure in FLIO is crucial for high-quality image acquisition and the possibility for systematic quantitative fluorescence lifetime analysis.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Augenklinik > Forschungsgruppe Augenheilkunde 04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Ophthalmology |
UniBE Contributor: |
Dysli, Chantal-Simone, Dysli, Denise-Muriel, Lincke, Joel-Benjamin, Jaggi, Damian, Wolf, Sebastian (B), Zinkernagel, Martin Sebastian |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
0275-004X |
Publisher: |
Lippincott, Williams & Wilkins |
Language: |
English |
Submitter: |
Chantal-Simone Dysli |
Date Deposited: |
12 Jan 2022 11:14 |
Last Modified: |
05 Dec 2022 15:57 |
Publisher DOI: |
10.1097/IAE.0000000000003235 |
PubMed ID: |
34111887 |
BORIS DOI: |
10.48350/162584 |
URI: |
https://boris.unibe.ch/id/eprint/162584 |
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