Stute, Petra; Ehrentraut, Stefan; Henneicke-von Zepelin, Hans-Heinrich; Nicken, Petra (2022). Gene expression analyses on multi-target mode of action of black cohosh in menopausal complaints - a pilot study in rodents. Archives of gynecology and obstetrics, 305(1), pp. 275-286. Springer-Verlag 10.1007/s00404-021-06105-8
|
Text
34259940_Stute2021_Article_GeneExpressionAnalysesOnMulti-.pdf - Published Version Available under License Creative Commons: Attribution (CC-BY). Download (1MB) | Preview |
PURPOSE
This study aimed at assessing gene expression profiles in hippocampus and hypothalamus of ovariectomized (OVX) rats with or without treatment with an isopropanolic extract of Cimicifuga racemosa rhizomes (iCR) in comparison to intact rats.
METHODS
Exploration of hippocampal (Hi) and hypothalamic (Hy) tissue from Sprague Dawley rats: without OVX (NHi = NHy = 4), tissues 3 months after OVX (NHi = 4, NHy = 3), or tissues of rats after their treatment with iCR for 3 months after OVX (NHi = NHy = 2). Gene expression profiles in these tissues were investigated by RNA-microarray-analysis and subsequent verification by qPCR.
RESULTS
4812 genes were differentially regulated when comparing the three groups in hippocampus and hypothalamus. iCR compensated the effects of OVX in 518 genes. This compensatory effect was most prominent in hippocampal signalling pathways, thereof genes (GAL, CALCA, HCRT, AVPR1A, PNOC, etc.) involved in thermoregulation, regulation of sleep and arousal, blood pressure regulation, metabolism, nociception, hormonal regulation, homeostasis, learning and cognition, mood regulation, neuroendocrine modulation, etc.. In the hypothalamus, iCR compensated OVX-effects at TAC3 and OPRM1 but not at KISS1. These genes are involved in the pathophysiology of hot flashes.
CONCLUSIONS
Our pilot study findings support a multifaceted mode of action of iCR in menopausal complaints on a tissue-specific brain gene expression level.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Department of Gynaecology, Paediatrics and Endocrinology (DFKE) > Clinic of Gynaecology |
UniBE Contributor: |
Stute, Petra |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
0932-0067 |
Publisher: |
Springer-Verlag |
Language: |
English |
Submitter: |
Monika Zehr |
Date Deposited: |
02 Feb 2022 09:04 |
Last Modified: |
05 Dec 2022 16:01 |
Publisher DOI: |
10.1007/s00404-021-06105-8 |
Related URLs: |
|
PubMed ID: |
34259940 |
Uncontrolled Keywords: |
Black cohosh Brain Cimicifuga racemosa Gene expression Menopausal symptoms Mode of action |
BORIS DOI: |
10.48350/163884 |
URI: |
https://boris.unibe.ch/id/eprint/163884 |
Actions (login required)
 |
Edit item |