Zhu, Jie; Han, Ling; Liu, Ruilai; Zhang, Zhenghua; Huang, Qiong; Fang, Xu; Yang, Ke; Huang, Guiqin; Zheng, Zhizhong; Yawalkar, Nikhil; Deng, Hui; Yan, Kexiang (2021). Identification of proteins associated with development of psoriatic arthritis in peripheral blood mononuclear cells: a quantitative iTRAQ-based proteomics study. Journal of translational medicine, 19(1), p. 331. BioMed Central 10.1186/s12967-021-03006-x
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BACKGROUND
Biomarkers for distinguishing psoriatic arthritis (PsA) from psoriasis without arthritis (PsO) are still lacking.
METHODS
We applied isobaric tags for relative and absolute quantification (iTRAQ) and LC-MS/MS to analyze the proteome profile of peripheral blood mononuclear cells (PBMCs) collected from patients with PsO, patients with PsA, and healthy controls. Bioinformatics analysis and western blotting were performed to identify and validate differentially expressed proteins.
RESULTS
We identified 389, 199, 291, and 60 significantly differentially expressed proteins (adj.p < 0.05) in the comparison of all psoriatic patients versus healthy controls, PsO group versus healthy controls, PsA group versus healthy controls, and PsA group versus PsO group, respectively. Among these proteins, 14 proteins may represent promising biomarkers for PsA: SIRT2, NAA50, ARF6, ADPRHL2, SF3B6, SH3KBP1, UBA3, SCP2, RPS5, NUDT5, NCBP1, SYNE1, NDUFB7, HTATSF1. Furthermore, western blotting confirmed that SIRT2 expression was significantly higher in PBMCs from PsA patients than PsO and healthy controls, and was negatively correlated with the phosphorylation of p38 mitogen-activated protein kinase (p-p38MAPK; p = 0.006, r = - 0.582).
CONCLUSIONS
This pilot study provided a broad characterization of the proteome of PBMCs in PsA as compared to PsO and healthy controls, which may help to provide prospective strategies for PsA diagnosis.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Department of Dermatology, Urology, Rheumatology, Nephrology, Osteoporosis (DURN) > Clinic of Dermatology |
UniBE Contributor: |
Yawalkar, Nikhil |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
1479-5876 |
Publisher: |
BioMed Central |
Language: |
English |
Submitter: |
Andrea Studer-Gauch |
Date Deposited: |
01 Feb 2022 10:23 |
Last Modified: |
05 Dec 2022 16:02 |
Publisher DOI: |
10.1186/s12967-021-03006-x |
PubMed ID: |
34344401 |
Uncontrolled Keywords: |
Biomarkers Peripheral blood Proteomics Psoriatic arthritis iTRAQ |
BORIS DOI: |
10.48350/164030 |
URI: |
https://boris.unibe.ch/id/eprint/164030 |
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