Schaer, Jessica; Cvetnic, Zeljko; Sukalic, Tomislav; Dörig, Sven; Grisiger, Martin; Iscaro, Carmen; Feliziani, Francesco; Pfeifer, Folke; Origgi, Francesco; Zanoni, Reto Giacomo; Abril, Carlos (2022). Evaluation of Serological Methods and a New Real-Time Nested PCR for Small Ruminant Lentiviruses. Pathogens, 11(2) MDPI AG 10.3390/pathogens11020129
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Small ruminant lentiviruses (SRLVs), i.e., CAEV and MVV, cause insidious infections with life-long persistence and a slowly progressive disease, impairing both animal welfare and productivity in affected herds. The complex diagnosis of SRLVs currently combines serological methods including whole-virus and peptide-based ELISAs and Immunoblot. To improve the current diagnostic protocol, we analyzed 290 sera of animals originating from different European countries in parallel with three commercial screening ELISAs, Immunoblot as a confirmatory assay and five SU5 peptide ELISAs for genotype differentiation. A newly developed nested real-time PCR was carried out for the detection and genotype differentiation of the virus. Using a heat-map display of the combined results, the drawbacks of the current techniques were graphically visualized and quantified. The immunoblot and the SU5-ELISAs exhibited either unsatisfactory sensitivity or insufficient reliability in the differentiation of the causative viral genotype, respectively. The new truth standard was the concordance of the results of two out of three screening ELISAs and the PCR results for serologically false negative samples along with genotype differentiation. Whole-virus antigen-based ELISA showed the highest sensitivity (92.2%) and specificity (98.9%) among the screening tests, whereas PCR exhibited a sensitivity of 75%.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Animal Pathology 05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology 05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) |
UniBE Contributor: |
Origgi, Francesco, Zanoni, Reto Giacomo, Abril Gaona, Carlos |
Subjects: |
600 Technology > 630 Agriculture |
ISSN: |
2076-0817 |
Publisher: |
MDPI AG |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
28 Feb 2022 15:16 |
Last Modified: |
05 Dec 2022 16:11 |
Publisher DOI: |
10.3390/pathogens11020129 |
PubMed ID: |
35215072 |
Uncontrolled Keywords: |
ELISA Maedi-Visna caprine arthritis-encephalitis diagnosis molecular methods nested real-time PCR serological methods small ruminant lentivirus |
BORIS DOI: |
10.48350/166124 |
URI: |
https://boris.unibe.ch/id/eprint/166124 |
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