Bossé, Janine T; Li, Yanwen; Cohen, Liza Miriam; Stegger, Marc; Angen, Øystein; Lacouture, Sonia; Gottschalk, Marcelo; Lei, Liancheng; Koene, Miriam; Kuhnert, Peter; Bandara, Aloka B; Inzana, Thomas J; Holden, Matthew T G; Harris, David; Oshota, Olusegun; Maskell, Duncan J; Tucker, Alexander W; Wren, Brendan W; Rycroft, Andrew N and Langford, Paul R (2021). Complete genome for Actinobacillus pleuropneumoniae serovar 8 reference strain 405: comparative analysis with draft genomes for different laboratory stock cultures indicates little genetic variation. Microbial genomics, 7(11) Microbiology Society 10.1099/mgen.0.000687
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We report here the complete genome sequence of the widely studied Actinobacillus pleuropneumoniae serovar 8 reference strain 405, generated using the Pacific Biosciences (PacBio) RS II platform. Furthermore, we compared draft sequences generated by Illumina sequencing of six stocks of this strain, including the same original stock used to generate the PacBio sequence, held in different countries and found little genetic variation, with only three SNPs identified, all within the degS gene. However, sequences of two small plasmids, pARD3079 and p405tetH, detected by Illumina sequencing of the draft genomes were not identified in the PacBio sequence of the reference strain.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) 05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology |
UniBE Contributor: |
Kuhnert, Peter |
Subjects: |
500 Science > 570 Life sciences; biology 600 Technology > 630 Agriculture |
ISSN: |
2057-5858 |
Publisher: |
Microbiology Society |
Language: |
English |
Submitter: |
Peter Kuhnert-Ryser |
Date Deposited: |
09 Mar 2022 10:52 |
Last Modified: |
05 Dec 2022 16:13 |
Publisher DOI: |
10.1099/mgen.0.000687 |
PubMed ID: |
34818145 |
Uncontrolled Keywords: |
Actinobacillus pleuropneumoniae SNPs serovar 8 reference genome |
BORIS DOI: |
10.48350/166759 |
URI: |
https://boris.unibe.ch/id/eprint/166759 |
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