Zuber, Benoît; Lučić, Vladan (2022). Neurons as a model system for cryo-electron tomography. Journal of structural biology: X, 6, p. 100067. Elsevier 10.1016/j.yjsbx.2022.100067
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Cryo-electron tomography (Cryo-ET) provides unique opportunities to image cellular components at high resolution in their native state and environment. While many different cell types were investigated by cryo-ET, here we review application to neurons. We show that neurons are a versatile system that can be used to investigate general cellular components such as the cytoskeleton and membrane-bound organelles, in addition to neuron-specific processes such as synaptic transmission. Furthermore, the synapse provides a rich environment for the development of cryo-ET image processing tools suitable to elucidate the functional and spatial organization of compositionally and morphologically heterogeneous macromolecular complexes involved in biochemical signaling cascades, within their native, crowded cellular environments.
Item Type: |
Journal Article (Review Article) |
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Division/Institute: |
09 Interdisciplinary Units > Microscopy Imaging Center (MIC) 04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy |
UniBE Contributor: |
Zuber, Benoît |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
2590-1524 |
Publisher: |
Elsevier |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
23 Mar 2022 09:43 |
Last Modified: |
05 Dec 2022 16:16 |
Publisher DOI: |
10.1016/j.yjsbx.2022.100067 |
PubMed ID: |
35310407 |
Uncontrolled Keywords: |
Cryo-electron tomography Electron microscopy Neurons Synapses |
BORIS DOI: |
10.48350/167852 |
URI: |
https://boris.unibe.ch/id/eprint/167852 |
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