Wanderer, Stefan; Grüter, Basil Erwin; Boillat, Gwendoline; Sivanrupan, Sivani; Rey, Jeannine; Catalano, Kristina; vonGunten, Michael; Widmer, Hans Rudolf; Andereggen, Lukas; Marbacher, Serge (2022). Parent artery-initiated and stent-mediated neointima formation in a rat saccular side wall model. Journal of neurointerventional surgery, 14(12), pp. 1258-1263. BMJ Publishing Group 10.1136/neurintsurg-2021-018297
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BACKGROUND
Unlike clipping that forms an immediate barrier of blood flow into intracranial aneurysms, endovascular treatments rely on thrombus organization and neointima formation. Therefore, a continuous endothelial cell layer is crucial to prevent blood flow in the former aneurysm. This study investigates the origin of endothelial cells in the neointima of endovascular treated aneurysms, specifically whether cells from the parent artery play a role in neointima formation.
METHODS
In male rats, decellularized and vital side wall aneurysms were treated by coil (n=16) or stent embolization (n=15). The cell tracer CM-Dil dye was injected into the clamped aorta before aneurysm suture to mark initial endothelial cells in the parent artery and enable tracking of their proliferation during follow-up. Aneurysms were analyzed for growth, thrombus formation, and recurrence. Histological evaluation followed with cell counts for specific regions-of-interest.
RESULTS
During follow-up, none of the 31 aneurysms ruptured. Macroscopic residual perfusion was observed in 12/16 rats after coiling and in 1/15 after stenting. Amounts of CM-Dil +cells in coiled versus stented decellularized aneurysms significantly decreased in the thrombus on day 7 (p=0.01) and neointima on day 21 (p=0.04). For vital aneurysms, the number of CM-Dil +cells in the neointima on day 21 showed no significant difference.
CONCLUSIONS
Healing patterns were worse in coil-treated than stent-treated aneurysms. Cell migration forming a neointima seemed mainly dependent on the adjacent vessel in decellularized aneurysms, but appeared buoyed by recruitment from aneurysm wall cells in vital aneurysms. Therefore, a cell-rich parent artery might be crucial.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Mu50 > Forschungsgruppe Neurochirurgie 04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Neurosurgery |
UniBE Contributor: |
Widmer, Hans Rudolf, Andereggen, Lukas |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
1759-8486 |
Publisher: |
BMJ Publishing Group |
Language: |
English |
Submitter: |
Nicole Söll |
Date Deposited: |
01 Jul 2022 16:39 |
Last Modified: |
05 Dec 2022 16:21 |
Publisher DOI: |
10.1136/neurintsurg-2021-018297 |
PubMed ID: |
35110397 |
Uncontrolled Keywords: |
aneurysm coil intervention stent vessel wall |
BORIS DOI: |
10.48350/171045 |
URI: |
https://boris.unibe.ch/id/eprint/171045 |
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