Tsioti, Ioanna; Liu, Xuan; Schwarzer, Petra; Zinkernagel, Martin S; Kokona, Despina (2022). Multimodal imaging of experimental choroidal neovascularization. International journal of ophthalmology, 15(6), pp. 886-893. IJO Press 10.18240/ijo.2022.06.05
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AIM
To compare choroidal neovascularization (CNV) lesion measurements obtained by in vivo imaging modalities, with whole mount histological preparations stained with isolectin GS-IB4, using a murine laser-induced CNV model.
METHODS
B6N.Cg-Tg(Csf1r-EGFP)1Hume/J heterozygous adult mice were subjected to laser-induced CNV and were monitored by fluorescein angiography (FA), multicolor (MC) fundus imaging and optical coherence tomography angiography (OCTA) at day 14 after CNV induction. Choroidal-retinal pigment epithelium (RPE) whole mounts were prepared at the end of the experiment and were stained with isolectin GS-IB4. CNV areas were measured in all different imaging modalities at day 14 after CNV from three independent raters and were compared to choroidal-RPE whole mounts. Intraclass correlation coefficient (ICC) type 2 (2-way random model) and its 95% confidence intervals (CI) were calculated to measure the correlation between different raters' measurements. Spearman's rank correlation coefficient (Spearman's r) was calculated for the comparison between FA, MC and OCTA data and histology data.
RESULTS
FA (early and late) and MC correlates well with the CNV measurements ex vivo with FA having slightly better correlation than MC (FA early Spearman's r=0.7642, FA late Spearman's r=0.7097, and MC Spearman's r=0.7418), while the interobserver reliability was good for both techniques (FA early ICC=0.976, FA late ICC=0.964, and MC ICC=0.846). In contrast, OCTA showed a poor correlation with ex vivo measurements (Spearman's r=0.05716) and high variability between different raters (ICC=0.603).
CONCLUSION
This study suggests that FA and MC imaging could be used for the evaluation of CNV areas in vivo while caution must be taken and comparison studies should be performed when OCTA is employed as a CNV monitoring tool in small rodents.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Augenklinik > Forschungsgruppe Augenheilkunde 04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Ophthalmology |
UniBE Contributor: |
Tsioti, Ioanna, Zinkernagel, Martin Sebastian, Kokona, Despina |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
2222-3959 |
Publisher: |
IJO Press |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
12 Jul 2022 14:34 |
Last Modified: |
05 Dec 2022 16:21 |
Publisher DOI: |
10.18240/ijo.2022.06.05 |
PubMed ID: |
35814883 |
Uncontrolled Keywords: |
choroidal neovascularization fluorescein angiography in vivo imaging multicolor fundus imaging optical coherence tomography angiography |
BORIS DOI: |
10.48350/171260 |
URI: |
https://boris.unibe.ch/id/eprint/171260 |
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