Pillet, Lauriane; Lim, David; Almulhim, Nourah; Benítez-Mateos, Ana I; Paradisi, Francesca (2022). Novel triple mutant of an extremophilic glycosyl hydrolase enables the rapid synthesis of thioglycosides. Chemical communications, 58(86), pp. 12118-12121. Royal Society of Chemistry 10.1039/D2CC04660A
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In order to expand the toolbox of enzymes available for thioglycoside synthesis, we describe here the first example of an extremophilic glycosyl hydrolase from Halothermothrix orenii (HorGH1) engineered towards thioglycosynthase activity with a novel combination of mutations. Using the triple mutant, HorGH1 M299R/E166A/E354G, a range of thioglycosides from glycosyl fluoride donors and aromatic thiols could be synthesised with exquisite stereoselectivity and good to excellent conversions (61-93%).
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
08 Faculty of Science > Department of Chemistry, Biochemistry and Pharmaceutical Sciences (DCBP) |
UniBE Contributor: |
Pillet, Lauriane, Lim, David, Benitez Mateos, Ana Isabel, Paradisi, Francesca |
Subjects: |
500 Science > 540 Chemistry |
ISSN: |
1359-7345 |
Publisher: |
Royal Society of Chemistry |
Funders: |
[42] Schweizerischer Nationalfonds ; [185] University of Bern: Seal of Excellence Fund |
Projects: |
Projects 19 not found. Projects 200021 not found. |
Language: |
English |
Submitter: |
Francesca Paradisi |
Date Deposited: |
11 Oct 2022 10:20 |
Last Modified: |
05 Dec 2022 16:26 |
Publisher DOI: |
10.1039/D2CC04660A |
BORIS DOI: |
10.48350/173654 |
URI: |
https://boris.unibe.ch/id/eprint/173654 |
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