In Vitro Effect of Instrumentation Using Ultrasonication with and without Hydrogen Peroxide on the Removal of Biofilms and Spread of Viable Microorganisms in Aerosols.

Stähli, Alexandra; Lanzrein, Carla; Milia, Egle; Sculean, Anton; Eick, Sigrun (2022). In Vitro Effect of Instrumentation Using Ultrasonication with and without Hydrogen Peroxide on the Removal of Biofilms and Spread of Viable Microorganisms in Aerosols. Oral health & preventive dentistry, 20(1), pp. 11-17. Quintessence 10.3290/j.ohpd.b2395059

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PURPOSE

To evaluate the use of hydrogen peroxide as an adjunct to ultrasonication (US) in biofilm removal and whether it can limit the spread of viable microorganisms in the aerosol.

MATERIALS AND METHODS

Multi-species biofilms were formed on dentin disks and titanium disks fixed on a plastic surface. After placing the specimens in a periodontal pocket model, an ultrasonic scaler was applied for 30 s, in part combined with 0.25% or 0.5% H2O2. After treatment, the remaining biofilm was analysed for bacterial counts (colony forming units [CFU]), biofilm quantity and metabolic activity. Further, the cytotoxic effect of hydrogen peroxide on periodontal ligament fibroblasts was assessed and the spread of bacteria in aerosol was quantified.

RESULTS

Ultrasonication reduced bacterial counts in biofilm, biofilm mass and metabolic activity on both dentin and titanium disks. Adjunctive use of 0.25% and 0.5% H2O2 more effectively reduced the viable bacteria in biofilm than ultrasonication alone; this was also found on both dentin and titanium. The different concentrations of H2O2 did not lead to corresponding differences in bacterial mass and metabolic activity. The spread of bacteria through aerosols was statistically significantly reduced when adjunctive H2O2 was used. However, a certain cytotoxic effect on periodontal ligament fibroblasts by H2O2 could not be ruled out.

CONCLUSIONS

Irrigating with H2O2 during periodontal instrumentation with an ultrasonic scaler increases the reduction of viable bacteria within biofilms. It might limit bacterial spreading via aerosols.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology
04 Faculty of Medicine > School of Dental Medicine > Periodontics Research

UniBE Contributor:

Stähli, Alexandra Beatrice, Lanzrein, Carla, Sculean, Anton, Eick, Sigrun

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1757-9996

Publisher:

Quintessence

Language:

English

Submitter:

Beatrix Margrit Stalder

Date Deposited:

02 Dec 2022 17:15

Last Modified:

05 Dec 2022 16:27

Publisher DOI:

10.3290/j.ohpd.b2395059

PubMed ID:

35049248

Uncontrolled Keywords:

aerosol biofilm hydrogen peroxide periodontal therapy

BORIS DOI:

10.48350/174299

URI:

https://boris.unibe.ch/id/eprint/174299

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