Fereig, Ragab M; Abdelbaky, Hanan H; Mazeed, Amira M; El-Alfy, El-Sayed; Saleh, Somaya; Omar, Mosaab A; Alsayeqh, Abdullah F; Frey, Caroline F (2022). Prevalence of Neospora caninum and Toxoplasma gondii Antibodies and DNA in Raw Milk of Various Ruminants in Egypt. Pathogens, 11(11) MDPI AG 10.3390/pathogens11111305
|
Text
pathogens-11-01305.pdf - Published Version Available under License Creative Commons: Attribution (CC-BY). Download (2MB) | Preview |
The prevalence of Neospora caninum and Toxoplasma gondii antibodies in raw milk samples was estimated in different ruminants and Egyptian governorates. Of 13 bulk milk samples tested by ELISA, five (38.5%) were positive for antibodies to N. caninum, and two samples were additionally positive for antibodies to T. gondii, resulting in a seroprevalence of 15.4% for both T. gondii and co-infection. In individual milk samples (n = 171) from the same bulks, antibodies to N. caninum were detected in 25.7%, to T. gondii in 14%, and 3.5% had antibodies to both parasites. A strong correlation between the OD values of the bulk samples and of the relevant individual milk samples was found for T. gondii (Pearson r = 0.9759) and moderately strong for N. caninum (Pearson r = 0.5801). Risk factor assessment for individual milk samples revealed that antibodies to T. gondii were significantly influenced by animal species, while no risk factors were detected for N. caninum antibodies. Additionally, DNA of N. caninum was detected in a bulk milk sample of cattle for the first time in Egypt, and DNA of T. gondii was found in bulk milk samples of cattle, sheep and goats. This is the first study in Egypt in which bulk milk samples of different ruminants were tested for the presence of N. caninum and T. gondii antibodies and DNA. Both individual and bulk milk samples are useful tools for monitoring antibody response to N. caninum and T. gondii infections in different ruminants in Egypt.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Parasitology 05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) |
UniBE Contributor: |
Frey Marreros Canales, Caroline Franziska |
Subjects: |
600 Technology > 630 Agriculture |
ISSN: |
2076-0817 |
Publisher: |
MDPI AG |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
16 Nov 2022 08:23 |
Last Modified: |
02 Mar 2023 23:36 |
Publisher DOI: |
10.3390/pathogens11111305 |
PubMed ID: |
36365056 |
Uncontrolled Keywords: |
ELISA PCR cattle neosporosis sheep toxoplasmosis |
BORIS DOI: |
10.48350/174751 |
URI: |
https://boris.unibe.ch/id/eprint/174751 |
Actions (login required)
 |
Edit item |