Besançon, Hervé; Larpin, Yu; Babiychuk, Viktoria S; Köffel, René; Babiychuk, Eduard B (2022). Engineered Liposomes Protect Immortalized Immune Cells from Cytolysins Secreted by Group A and Group G Streptococci. Cells, 11(1) MDPI 10.3390/cells11010166
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The increasing antibiotic resistance of bacterial pathogens fosters the development of alternative, non-antibiotic treatments. Antivirulence therapy, which is neither bacteriostatic nor bactericidal, acts by depriving bacterial pathogens of their virulence factors. To establish a successful infection, many bacterial pathogens secrete exotoxins/cytolysins that perforate the host cell plasma membrane. Recently developed liposomal nanotraps, mimicking the outer layer of the targeted cell membranes, serve as decoys for exotoxins, thus diverting them from attacking host cells. In this study, we develop a liposomal nanotrap formulation that is capable of protecting immortalized immune cells from the whole palette of cytolysins secreted by Streptococcus pyogenes and Streptococcus dysgalactiae subsp. equisimilis-important human pathogens that can cause life-threatening bacteremia. We show that the mixture of cholesterol-containing liposomes with liposomes composed exclusively of phospholipids is protective against the combined action of all streptococcal exotoxins. Our findings pave the way for further development of liposomal antivirulence therapy in order to provide more efficient treatment of bacterial infections, including those caused by antibiotic resistant pathogens.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy 04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy > Cell Biology |
UniBE Contributor: |
Besançon, Hervé, Larpin, Yu-Noël, Babiychuk, Viktoriia, Köffel, René, Babiichuk, Eduard |
Subjects: |
500 Science > 570 Life sciences; biology 600 Technology > 610 Medicine & health |
ISSN: |
2073-4409 |
Publisher: |
MDPI |
Language: |
English |
Submitter: |
Hervé Besançon |
Date Deposited: |
29 Nov 2022 18:02 |
Last Modified: |
25 Apr 2023 14:35 |
Publisher DOI: |
10.3390/cells11010166 |
PubMed ID: |
35011729 |
Uncontrolled Keywords: |
Streptococcus antibiotic resistance antivirulence bacterial infection immune cells liposome nanotrap toxin |
BORIS DOI: |
10.48350/175234 |
URI: |
https://boris.unibe.ch/id/eprint/175234 |
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