Dysli, Chantal; Dysli, Denise; Wolf, Sebastian; Zinkernagel, Martin (2023). Fluorescence lifetime distribution in phakic and pseudophakic healthy eyes. PLoS ONE, 18(1), e0279158. Public Library of Science 10.1371/journal.pone.0279158
|
Text
journal.pone.0279158.pdf - Published Version Available under License Creative Commons: Attribution (CC-BY). Download (2MB) | Preview |
PURPOSE
To investigate the influence of the lens status and to describe fundus autofluorescence lifetimes (FLT) in a large cohort of healthy eyes across a wide age range.
MATERIALS AND METHODS
FLT data were acquired from healthy phakic and pseudophakic eyes using fluorescence lifetime imaging ophthalmoscopy (FLIO). Retinal autofluorescence was excited with a 473 nm laser and emitted autofluorescence was detected in a short and a long spectral channel (SSC: 498-560 nm; LSC: 560-720 nm).
RESULTS
141 healthy eyes from 141 participants (56 ± 18 years) were included. The shortest mean FLTs were measured within the macular center, followed by the temporal inner and outer ETDRS (Early Treatment Diabetic Retinopathy Study) grid segments, and the remaining areas of the inner and the outer ETDRS ring. In phakic participants (81%), mean, short and long FLTs correlated with the age (SSC: r2 = 0.54; LSC: r2 = 0.7; both p<0.0001) with an increase of about 33 ps in the SSC resp. 28 ps in the LSC per decade. In pseudophakic subjects (19%), mean FLTs only correlated with age in the long spectral channel (r2 = 0.44; p = 0.0002) but not in the short spectral channel (r2 = 0.066; p = 0.2).
CONCLUSIONS
Fundus autofluorescence lifetimes are age dependent. FLTs in the SSC are more susceptible to lens opacities but less dependent on age changes, whereas FLTs in the LSC are largely independent of the lens status but display a higher degree of age dependency.
STUDY REGISTRY
ClinicalTrials.gov NCT01981148.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Augenklinik > Forschungsgruppe Augenheilkunde 04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Ophthalmology |
UniBE Contributor: |
Dysli, Chantal-Simone, Dysli, Denise-Muriel, Wolf, Sebastian (B), Zinkernagel, Martin Sebastian |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
1932-6203 |
Publisher: |
Public Library of Science |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
10 Jan 2023 16:32 |
Last Modified: |
15 Jan 2023 02:19 |
Publisher DOI: |
10.1371/journal.pone.0279158 |
PubMed ID: |
36608033 |
BORIS DOI: |
10.48350/177011 |
URI: |
https://boris.unibe.ch/id/eprint/177011 |
Actions (login required)
 |
Edit item |