Wiedmer, Linda (2018). Biomedical applications of silica nanoparticles in the brain. Little helpers - with or without risks? (Unpublished). (Dissertation, Vetsuisse)
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Nanomaterials offer promising tools for medical applications including neurophar-macology. Nanoparticles (NPs) are used for drug delivery and biodegradable im-plants in the central nervous system. Immediate or sustained release of NPs in the brain may pose a hazard. Previous studies demonstrated that silica-Ɛ-polycaprolactone-nanoparticles (SiPCL-NPs) were taken up by neuron-like SH-SY5Y cells and led to a reduction in neuronal differentiation. In this study, effects of SiPCL-NPs were examined in SH-SY5Y cells differentiated with all-trans retinoic acid and staurosporine (ST). Resulting dopaminergic and cholinergic phenotypes were analyzed at the end of differentiation with NPs given at various time during differentiation. A significant downregulation of tyrosine hydroxylase was found in ST-differentiated cells when NPs were added in the middle and at the end of differ-entiation, indicating a loss of dopaminergic neurons. The expression of the cholin-ergic marker, high-affinity choline transporter, was decreased when SiPCL-NPs were given before the differentiation. NP-exposure did not alter markers of signaling pathways related to differentiation (Akt, MAP-K, MAP-2) and neurodegeneration (β-catenin, GSK-3β). The data indicated that SiPCL-NPs alter the number of dopamin-ergic and cholinergic cells but did not affect pathways involved in neuronal differen-tiation and degeneration.
Item Type: |
Thesis (Dissertation) |
|---|---|
Division/Institute: |
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Veterinary Pharmacology and Toxicology |
UniBE Contributor: |
Wiedmer, Linda |
Subjects: |
500 Science > 570 Life sciences; biology |
Language: |
English |
Submitter: |
Angélique Ducray |
Date Deposited: |
02 Feb 2023 09:01 |
Last Modified: |
02 Feb 2023 23:27 |
Uncontrolled Keywords: |
nanomedicine, silica nanoparticles, neuronal differentiation, neurodegeneration, SH-SY5Y cells |
BORIS DOI: |
10.48350/178202 |
URI: |
https://boris.unibe.ch/id/eprint/178202 |
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