Aaldijk, Dea; Reusser, Adrian; Eppler, Elisabeth; Yao, Eveline; Djonov, Valentin; Halm, Sebastian (2024). Morphological changes in striated muscle fibers caused by components of the Thiel embalming method. Folia morphologica, 83(1), pp. 83-91. Via Medica 10.5603/FM.a2023.0010
Full text not available from this repository.BACKGROUND
Thiel-fixed body donors are highly valued for surgical training courses. The pronounced flexibility of Thiel-fixed tissue has been postulated to be caused by histologically visible fragmentation of striated muscle. The aim of this study was to analyze whether a specific ingredient, pH, decay, or autolysis could cause this fragmentation in order to modulate the Thiel solution to adapt specimen flexibility specifically to the needs of different courses.
MATERIALS AND METHODS
Striated muscle of the mouse was fixed for different time periods in formalin, Thiel solution, and its individual ingredients, and analysed by light microscopy. Further, pH-values of Thiel solution and its ingredients were measured. In addition, unfixed muscle tissue was histologically analyzed including Gram staining to investigate a relationship between autolysis, decomposition, and fragmentation.
RESULTS
Muscle fixed with Thiel solution for three months was slightly more fragmentated than muscle fixed for 1 day. Fragmentation was more pronounced after one year of immersion. Three individual salt ingredients showed slight fragmentation. Decay and autolysis had no effect on fragmentation, which occurred regardless of the pH of all solutions.
CONCLUSIONS
Fragmentation of Thiel-fixed muscle is dependent on fixation time and most likely occurs due to salts present in the Thiel solution. Adjustment of the salt composition in the Thiel solution with verification of the influence on the fixation effect, fragmentation and flexibility of the cadavers could be performed in further studies.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy 04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy > Topographical and Clinical Anatomy |
UniBE Contributor: |
Aaldijk, Dea, Reusser, Adrian, Eppler, Elisabeth, Yao, Eveline, Djonov, Valentin Georgiev, Halm, Sebastian |
Subjects: |
600 Technology > 610 Medicine & health 500 Science > 570 Life sciences; biology |
ISSN: |
1644-3284 |
Publisher: |
Via Medica |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
23 Feb 2023 09:08 |
Last Modified: |
03 Apr 2024 00:11 |
Publisher DOI: |
10.5603/FM.a2023.0010 |
PubMed ID: |
36811140 |
Uncontrolled Keywords: |
boric acid formalin fragmentation light microscopy pH saturated salt solution skeletal muscle tissue fixation |
URI: |
https://boris.unibe.ch/id/eprint/179041 |
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